The Mouse B- raf Gene Encodes Multiple Protein Isoforms with Tissue-specific Expression
| Autor: | Georges Calothy, Odile Lecoq, Catherine Papin, Alain Eychène, Jean-Vianney Barnier |
|---|---|
| Přispěvatelé: | PERIGNON, Alain, CNRS UMR 146 - Institut Curie - Developmental Genetics of Melanocytes (CNRS), Centre National de la Recherche Scientifique (CNRS), Signalisation normale et pathologique de l'embryon aux thérapies innovantes des cancers, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Male
MESH: Signal Transduction Gene Expression MESH: Amino Acid Sequence MESH: Base Sequence Proto-Oncogene Mas Biochemistry MAP2K7 Mice Exon 0302 clinical medicine Antibody Specificity Tissue Distribution MESH: Animals Peptide sequence 0303 health sciences MESH: Alternative Splicing Exons 030220 oncology & carcinogenesis RNA splicing MESH: Birds [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Tandem exon duplication Signal Transduction Gene isoform MESH: DNA Primers DNA Complementary MESH: Gene Expression Molecular Sequence Data Protein Serine-Threonine Kinases Biology MESH: Protein-Serine-Threonine Kinases Birds 03 medical and health sciences Exon trapping Proto-Oncogene Proteins [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Proto-Oncogenes Animals Humans Amino Acid Sequence RNA Messenger MESH: Tissue Distribution MESH: Antibody Specificity Molecular Biology MESH: Mice DNA Primers 030304 developmental biology MESH: RNA Messenger MESH: Molecular Sequence Data MESH: Humans Base Sequence Alternative splicing Cell Biology MESH: DNA Complementary Molecular biology MESH: Male Proto-Oncogene Proteins c-raf MESH: Proto-Oncogene Proteins Alternative Splicing MESH: Proto-Oncogenes MESH: Proto-Oncogene Proteins c-raf MESH: Exons |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, 1995, 270 (40), pp.23381-23389. ⟨10.1074/jbc.270.40.23381⟩ Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1995, 270 (40), pp.23381-23389. ⟨10.1074/jbc.270.40.23381⟩ Scopus-Elsevier |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.270.40.23381 |
| Popis: | International audience; The c-Rmil/B-raf proto-oncogene is a member of the mil/raf family encoding serine/threonine protein kinases shown to be involved in signal transduction from the membrane to the nucleus. We isolated from a mouse brain library B-raf cDNAs containing a previously unidentified 36-base pair alternatively spliced exon located between exons 8 and 9 and, therefore, designated exon 8b. Human and mouse B-raf mRNAs also contain the 120-base pair alternatively spliced exon 10 previously described in the avian c-Rmil gene. Independent splicing of these two exons, located between the conserved region 2 (CR2) and the catalytic domain (CR3) gives rise to mRNAs potentially encoding four distinct proteins. By using specific sera generated against different portions of B-Raf, we identified at least 10 protein isoforms in adult mouse tissues. Some isoforms, in the range of 69-72 kDa, are not recognized by antisera directed against peptides encoded by exons 1 and 2, indicating the existence of B-Raf proteins with two different NH2 extremities. The other isoforms, in the range of 79-99 kDa, contain the amino acids encoded by exons 1 and 2, by either or both of the alternatively spliced exons, and, possibly, by another of the unidentified exon. Analysis of B-raf mRNA expression by reverse transcriptase-polymerase chain reaction and immunocharacterization of B-Raf proteins in different tissues of the adult mouse showed a tissue-specific pattern of B-Raf isoforms expression. Interestingly, isoforms containing amino acids encoded by exon 10 are specifically expressed in neural tissues. Taken together, these results suggest that distinct B-Raf proteins could be involved, in a tissue-specific manner, in signal transduction pathways. |
| Databáze: | OpenAIRE |
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