Cytokine expression in the gut associated lymphoid tissue after oral administration of attenuated Salmonella vaccine strains
| Autor: | Sivadasan Kanangat, Kevin L. Karem, Barry T. Rouse |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Salmonella typhimurium
Salmonella Gut-associated lymphoid tissue medicine.medical_treatment Administration Oral Biology Vaccines Attenuated medicine.disease_cause Microbiology Proinflammatory cytokine Mice Peyer's Patches Immune system Antigen Immunity medicine Animals Mesentery Mice Inbred BALB C General Veterinary General Immunology and Microbiology Typhoid-Paratyphoid Vaccines Public Health Environmental and Occupational Health Salmonella vaccine Infectious Diseases medicine.anatomical_structure Cytokine Immunology Cytokines Molecular Medicine Female Lymph Nodes |
| Zdroj: | Vaccine. 14:1495-1502 |
| ISSN: | 0264-410X |
| Popis: | The use of attenuated Salmonella vaccine vectors as delivery vehicles for heterologous antigens has been extensive. The majority of work has analyzed the specific immune responses to the recombinant antigen in question. In addition, most analysis has been performed on animals maintained with sterile food, water, and bedding. This report defines the Salmonella specific cytokine responses in the gut associated lymphoid tissue after oral immunization with two highly publicized attenuated Salmonella carrier strains in animals maintained with nonsterile food, water, and bedding. Increases in expression of both IL-4 and IFN-gamma occur following immunization with either Salmonella construct. In addition, other cytokines (IL-6, IL-7, IL-12) increase at similar levels in either BRD509 or KR1 dosed animals. Proinflammatory cytokines IL-1 and TNF-alpha are also present but unchanged at early time points (6, 24, and 72 hours), increasing only after 7 days postimmunization. These data have implications in the generation of immunity to recombinant antigens since the response to Salmonella will dictate the direction of responses in terms of CD4 T cell activation. |
| Databáze: | OpenAIRE |
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