Larix decidua Bark as a Source of Phytoconstituents: An LC-MS Study
| Autor: | Gabriele De Nadai, Valeria Baldan, Marta Faggian, Gianni Baratto, Stefania Sut, Elena Dalla Gassa, Stefano Dall'Acqua, Stefano Francescato, Sara Ferrari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Phytochemicals Pharmaceutical Science Larix 01 natural sciences Article Antioxidants Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound Nutraceutical lcsh:Organic chemistry Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Drug Discovery medicine Organic chemistry Proanthocyanidins Physical and Theoretical Chemistry polyphenols LC-MS Larix decidua bark flavonoids procyanidins spiro-polyphenol biology Traditional medicine Molecular Structure 010405 organic chemistry Chemistry Organic Chemistry Extraction (chemistry) biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry Chemistry (miscellaneous) Polyphenol visual_art visual_art.visual_art_medium Plant Bark Molecular Medicine Bark Larch Chromatography Liquid |
| Zdroj: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 22; Issue 11; Pages: 1974 Molecules, Vol 22, Iss 11, p 1974 (2017) |
| ISSN: | 1420-3049 |
| Popis: | Larix decidua bark is a waste of the timber industry and is widely diffused in Northern Italy. This material can be considered a good source of antioxidants and phytoconstituents with possible use in cosmetic or nutraceutical products. In this study, simple extraction of larch bark was performed using mixtures of ethanol/water. Furthermore, the phytochemical composition of larch bark extract was studied using LC-MSn methods and the main constituents were identified as flavonoids, spiro-polyphenols, and procyanidins. To confirm the identification by LC-MS semi-preparative HPLC was performed in order to isolate the main constituents and verify the structures by ¹H-NMR. Antioxidant properties were studied using an in vitro approach combining DPPH assay and LC-MS in order to establish different roles of the various classes of phytochemicasl of the extract. DPPH activity of some of the isolated compounds was also assessed. The overall results indicate this waste material as a good source of antioxidant compounds, mainly procyanidins, whichresulted the most active constituents in the DPPH assay. |
| Databáze: | OpenAIRE |
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