Direct interaction of C/EBPdelta and Sp1 at the GC-enriched promoter region synergizes the IL-10 gene transcription in mouse macrophage
| Autor: | Yi-Wen Liu, Ben Kuen Chen, Ju Ming Wang, Ben Tzu Chiang, Wen Chang Chang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
CCAAT-Enhancer-Binding Protein-delta
Leucine zipper Transcription Genetic Sp1 Transcription Factor Endocrinology Diabetes and Metabolism Clinical Biochemistry Biology Mice Gene expression Protein Interaction Mapping Animals Pharmacology (medical) Binding site Promoter Regions Genetic Molecular Biology Transcription factor Regulation of gene expression ATF3 Ccaat-enhancer-binding proteins Macrophages Biochemistry (medical) Promoter Cell Biology General Medicine Molecular biology GC Rich Sequence Interleukin-10 Protein Structure Tertiary |
| Zdroj: | Journal of biomedical science. 13(5) |
| ISSN: | 1021-7770 |
| Popis: | We previously reported that LPS activates the transcription of the IL-10 gene through the Sp1 and C/EBP binding sites and indicated that Sp1, C/EBPbeta and C/EBPdelta can coactivate the IL-10 gene expression in mouse macrophage cells [Liu Y.-W., Tseng H.-P., Chen L.-C., Chen B.-K., Chang W.-C. J. Immunol. 171: 821-828, 2003]. In the present report, we demonstrated the direct physical interaction between C/EBPdelta and Sp1, and also mapped the interaction domains of these two proteins. C/EBPdelta binds to Sp1 via its basic region leucine zipper domain. The C-terminus of Sp1 was also the major region interacting with C/EBPdelta. However, both glutamine- and serine/threonine-rich homologus regions of Sp1 also interacted with C/EBPdelta. The binding of Sp1 and C/EBPdelta as a complex to the Sp1 binding site on the promoter of IL-10 was further confirmed by using the DNA affinity precipitation assay. By using Sp1-deficient SL2 cells, we also found that the overexpressions of C/EBPdelta and Sp1 synergically activate the transcriptional activity of IL-10 gene. Taken together, our present results revealed a novel mechanism of a superactivation of Sp1 by C/EBPdelta via a direct interaction between these two transcription factors leading to the activation of the IL-10 gene in mouse macrophage cells. |
| Databáze: | OpenAIRE |
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