Cytokine Profiles in Eye Muscle Tissue and Orbital Fat Tissue from Patients with Thyroid-Associated Ophthalmopathy*
| Autor: | Dame Yang, Ikuyo Miyake, Yuji Hiromatsu, Kyohei Nonaka, Yoichi Inoue, Tomasz Bednarczuk |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Endocrinology Diabetes and Metabolism Graves' disease medicine.medical_treatment Clinical Biochemistry Adipose tissue Biology Biochemistry Endocrinology Internal medicine Gene expression medicine Humans RNA Messenger Aged Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) Interleukin Orbitalis muscle Middle Aged medicine.disease Graves Disease Cytokine Adipose Tissue Gene Expression Regulation Oculomotor Muscles Cytokines Female Tumor necrosis factor alpha Orbit |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 85:1194-1199 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.85.3.6433 |
| Popis: | Eye muscle (EM) and retroorbital fat tissue are two major sites of involvement in thyroid-associated ophthalmopathy (TAO). Lymphocytic infiltration in these tissues is a prominent histological feature of TAO. We have investigated the cytokine gene profiles in EM and orbital fat (OF) tissues from patients with TAO. Total RNA was isolated from EM tissue of 14 patients and from OF tissues of 29 patients with TAO. Cytokine gene expression was assessed by RT-PCR using paired primers for interferon gamma (IFNgamma), tumor necrosis factor alpha (TNFalpha), interleukin (IL)-1beta, IL-2, IL-4, IL-6, IL-10, CD4, CD8, and glyceraldehyde-3-phosphate dehydrogenase. IFNgamma, TNFalpha, IL-1beta, and IL-6 messenger RNA (mRNA) were mainly detected in EM tissue, whereas IL-4 and IL-10 mRNA were detected in only one patient. On the other hand, in OF tissue, IL-4 and IL-10 mRNA were detected in 24% and 38% of the patients, respectively, and IFNgamma, IL-1beta, and IL-6 mRNA were less often detected compared with EM tissue. The enlargement of EM tissue as assessed by computed tomography correlated significantly with TNFalpha mRNA expression in EM tissue. The orbital volume was positively correlated with IL-6 mRNA expression and negatively correlated with IL-4 mRNA and IL-10 mRNA expression in OF tissue. These results suggest that T helper (Th) 1-like cytokines predominate in EM tissue in most patients and that the predominant cytokine profile in OF tissue varies from patient to patient. Both Th1-like and Th2-like immune responses may play roles in the development of two components of ophthalmopathy. |
| Databáze: | OpenAIRE |
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