Chiral Analysis of Methamphetamine in Oral Fluid Samples: A Method to Distinguish Licit from Illicit Drug Use
| Autor: | Damon Borg, Cindy Lantigua, Richard Stripp, Elizabeth Kolb |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Accuracy and precision
Health Toxicology and Mutagenesis Amphetamine-Related Disorders Pharmacology Toxicology Tandem mass spectrometry 01 natural sciences Analytical Chemistry Methamphetamine 03 medical and health sciences Structure-Activity Relationship 0302 clinical medicine Isomerism Tandem Mass Spectrometry medicine Environmental Chemistry Humans 030216 legal & forensic medicine Solid phase extraction Chiral derivatizing agent Saliva MAMP Chromatography Reverse-Phase Chemical Health and Safety Chromatography Alanine Chemistry fungi 010401 analytical chemistry Solid Phase Extraction Reproducibility of Results Reversed-phase chromatography Reference Standards 0104 chemical sciences Substance Abuse Detection Dinitrobenzenes Calibration Linear Models Central Nervous System Stimulants Enantiomer Drug Monitoring medicine.drug |
| Zdroj: | Journal of analytical toxicology. 42(1) |
| ISSN: | 1945-2403 |
| Popis: | Methamphetamine (MAMP) is a popular illicit drug abused for its central nervous system stimulating effects. MAMP is also used therapeutically in the treatment of overeating disorders, narcolepsy, attention deficit disorder, in over-the-counter (OTC) products to ease nasal congestion. MAMP exists in two enantiomeric forms, dextrorotary (d-MAMP) or levorotary (l-MAMP). The compounds are similar in chemical structure, simply differing in the orientation of functional groups around the asymmetric carbon. In part because of the availability of l-MAMP in OTC nasal inhalers, forensic guidelines require a sample to contain greater than 20% d-MAMP to consider illicit drug use when interpreting results. Standard analytical methods readily detect MAMP in biological specimens but cannot resolve the enantiomeric composition of the sample. Specialized analytical techniques based on chiral separation of the enantiomers are required to differentiate d-MAMP from l-MAMP. Our laboratory sought to develop and validate a method for the analysis of oral fluid specimens for d/l-MAMP using a chiral derivatizing agent and traditional reverse phase liquid chromatography tandem mass spectrometry (LC-MS-MS). MAMP was extracted from dilute oral fluid samples using Strata-XC solid phase extraction (SPE) cartridges and derivatized with Marfey's reagent. Chromatographic separation was achieved using Zorbax Eclipse Plus C18 columns. Linearity, accuracy and precision, recovery, matrix effects and specificity of the method were all within acceptable criteria. Intraday accuracy ranged from 93.3 to 103.4% and precision 0.1 to 1.6%. Interday accuracy ranged from 90.0 to 103.4% and precision 3.8 to 11.6%. Finally, having previously tested positive for MAMP using non-chiral analysis, 256 de-identified authentic oral fluid samples were analyzed using this validated method. 98% of all samples tested positive for d-MAMP at greater than 20%. |
| Databáze: | OpenAIRE |
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