Nitric oxide donor-mediated killing of bioluminescent Escherichia coli
| Autor: | Marko Virta, Pauli I. Vuorinen, Matti Karp |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
medicine.disease_cause
Nitric Oxide Nitric oxide chemistry.chemical_compound medicine Escherichia coli Bioluminescence Pharmacology (medical) Luciferase Viability assay Cloning Molecular Cytotoxicity Luciferases Pharmacology biology Triazoles biology.organism_classification Enterobacteriaceae Infectious Diseases Biochemistry chemistry Molsidomine Luminescent Measurements Light emission Research Article |
| Popis: | The antimicrobial activities of two nitric oxide-releasing compounds against Escherichia coli were investigated by using recombinant E. coli cloned with a luciferase gene from Pyrophorus plagiophthalamus. Since luciferase uses intracellular ATP to generate visible light which can be measured from living cells in real time, we wanted to compare the extent to which cell viability parallels light emission. Results from luminescence measurements and CFU counts were in good agreement, and the decrease in light emission was shown to provide a rapid and more sensitive indication of cytotoxicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|