Cholecystokinin (CCK) Receptor and CCK Gene Expression in Human Pituitary Adenomas and in vitro Effects of CCK Peptides on GH and Gonadotrophin Secretion
| Autor: | Eric F. Adams, Eftychia Oikonomou, Michael Buchfelder, Charlton A |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adenoma
Adult Male Cholagogues and Choleretics medicine.medical_specialty Endocrinology Diabetes and Metabolism Neuropeptide Biology digestive system Cholecystokinin receptor Paracrine signalling Endocrinology Anterior pituitary Internal medicine Gene expression Tumor Cells Cultured Internal Medicine medicine Humans Pituitary Neoplasms Receptor Autocrine signalling Aged Cholecystokinin digestive oral and skin physiology General Medicine Middle Aged Receptor Cholecystokinin B Neoplasm Proteins Receptor Cholecystokinin A Gene Expression Regulation Neoplastic Autocrine Communication medicine.anatomical_structure Growth Hormone Female Peptides Gonadotropins hormones hormone substitutes and hormone antagonists |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 115:683-689 |
| ISSN: | 1439-3646 0947-7349 |
| DOI: | 10.1055/s-2007-985362 |
| Popis: | There is growing evidence that cholecystokinin (CCK) affects growth and differentiation of anterior pituitary cells, via the CCK-B receptor. The possibility of an autocrine / paracrine role for CCK to modulate hormone secretion in human pituitary tumour cells is demonstrated here by RT-PCR and direct sequencing. In support of this conclusion, a neutralising antibody against the CCK peptide exhibited a dose dependent inhibition of hormone secretion by functionless pituitary adenomas. Total RNA was extracted from human pituitary adenomas, reverse transcribed into cDNA and subjected to PCR using primers specific for the gene for CCK, CCK-A and CCK-B receptors. PCR bands of the predicted length were observed in all tumours using human CCK gene and CCK-B receptor primers. Restriction digestion and direct sequence analysis provided further evidence that they represented both the human CCK peptide along with the CCK-A and/B receptor mRNA. CCK-33 and CCK octapeptide sulphate (CCK-8s) both powerfully stimulated phosphatidylinositol hydrolysis, providing evidence for functional activity of the CCK-A and/B receptors. A direct stimulatory effect of CCK peptides on both LH and FSH secretion is reported for the first time, whereas stimulatory effects on GH were blocked by antagonists to CCK. These results may indicate an autocrine role for CCK in the functioning and perhaps development of human pituitary tumours. © J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart. |
| Databáze: | OpenAIRE |
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