Coaxial printing of double-layered and free-standing blood vessel analogues without ultraviolet illumination for high-volume vascularised tissue
| Autor: | Thi Thao Dang, Chang Ho Hwang, Kyo-in Koo, Sung Hoon Back, Van Thuy Duong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Materials science Angiogenesis 0206 medical engineering Biomedical Engineering Shell (structure) Bioengineering 02 engineering and technology Biochemistry Umbilical vein Biomaterials Extracellular matrix Monolayer Human Umbilical Vein Endothelial Cells Humans Lighting Tissue Engineering Tissue Scaffolds Bioprinting General Medicine 021001 nanoscience & nanotechnology 020601 biomedical engineering Printing Three-Dimensional 0210 nano-technology Layer (electronics) Type I collagen Biotechnology Biomedical engineering |
| Zdroj: | Biofabrication. 12(4) |
| ISSN: | 1758-5090 |
| Popis: | Human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HASMCs) were coaxially and continuously extruded without ultraviolet illumination using a microfluidic-based nozzle. Type I collagen (3 mg ml−1) containing HUVECs and a crosslinking reagent (100 mM CaCl2) were supplied as the core material. A mixture of 3 mg ml−1 of type I collagen (25%) and 1.8% weight volume−1 of sodium alginate (75%) was provided as the shell layer material surrounding the core material. The HUVECs were well proliferated at the core and reshaped into a monolayer formation along the axial direction of the scaffold. The HASMCs showed more than 90% cell viability in the shell layer. Fluorescent beads were passed through the inside channel of the scaffold with the HUVEC core and HASMC shell using an in-house connector. This double-layered scaffold showed higher angiogenesis in growth factor-free medium than the scaffold with only a HUVEC core. The HASMCs in the shell layer affected angiogenesis, extracellular matrix secretion, and outer diameter. The proposed technique could be applied to three-dimensional bioprinting for the production of high-volume vascularised tissue. |
| Databáze: | OpenAIRE |
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