A SCAR marker for the analysis of chloroplast DNA from different cultivars of Cornus officinalis
Autor: | Lu Zhang, Junhan Shi, Li J, Bingya Kang, Zhang T, Jinjin Wang, Chen Sq, Ruixin Liu |
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Rok vydání: | 2015 |
Předmět: |
Genetic Markers
Germplasm Polymorphism Genetic Base Sequence biology Molecular Sequence Data DNA Chloroplast food and beverages Sequence Analysis DNA General Medicine Cornus officinalis biology.organism_classification Random Amplified Polymorphic DNA Technique RAPD Cornus Transformation Genetic Chloroplast DNA Genetic distance Genetic marker Botany Officinalis Genetics Cluster Analysis Cultivar Molecular Biology |
Zdroj: | Genetics and Molecular Research. 14:17170-17181 |
ISSN: | 1676-5680 |
Popis: | The aims of this study were to establish a random amplified polymorphic DNA (RAPD) fingerprint database of chloroplast DNA (cpDNA) from different cultivars of Cornus officinalis and to convert RAPD markers to sequence characterized amplified regions (SCAR) markers. A method of extraction was established that was suitable for obtaining cpDNA from samples rapidly dried in silicone; an RAPD fingerprint database was built; and the genetic distance between samples was used as statistical clustering variables for calculating DICE genetic similarity coefficients and for building a kinship tree chart. RAPD markers were converted to SCAR markers to design specific primers, and samples from C. officinalis cultivars, plants of the same family, and its adulterants, were used for amplification and identification. Fifteen amplified primers with stable polymorphisms were screened for amplification of 130 copies of materials. In total, 57 sites were achieved, 40 of which were polymorphic, and the polymorphic rate was up to 70.18%. A genetic tree was built based on seven cultivars. SCAR markers of C. officinalis cpDNA were successfully converted into RAPD markers. cpDNA samples from hawthorn, C. officinalis, Cornus wood, and grape were used for SCAR amplification, and their bands were distinctly different. In conclusion, SCAR markers and cpDNA may be used for research on C. officinalis and its adulterants, and the results may provide a basis for identifying germplasm and screening fine varieties at a molecular level. |
Databáze: | OpenAIRE |
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