Stromal cell-derived factor 1-mediated CXCR4 signaling in rat and human cortical neural progenitor cells
| Autor: | Jialin C. Zheng, Nobutaka Fujii, Hirokazu Tamamura, Yunlong Huang, Hui Peng, David Erichsen, Jeremy Rose, Shelley Herek |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Receptors CXCR4 Stromal cell Blotting Western Cell Separation Biology Pertussis toxin Cellular and Molecular Neuroscience Chemokine receptor Internal medicine otorhinolaryngologic diseases medicine Cyclic AMP Animals Humans Inosine Triphosphate Stromal cell-derived factor 1 Protein kinase B Cerebral Cortex Neurons Chemotaxis Stem Cells Neurogenesis Cell Polarity Immunohistochemistry Neural stem cell Chemokine CXCL12 Cell biology Rats Enzyme Activation stomatognathic diseases Endocrinology biology.protein Cytokines Calcium Signal transduction Mitogen-Activated Protein Kinases Chemokines CXC Signal Transduction |
| Zdroj: | Journal of neuroscience research. 76(1) |
| ISSN: | 0360-4012 |
| Popis: | Stromal cell-derived factor 1 (SDF-1) and the chemokine receptor CXCR4 are highly expressed in the nervous system. Knockout studies have suggested that both SDF-1 and CXCR4 play essential roles in cerebellar, hippocampal, and neocortical neural cell migration during embryogenesis. To extend these observations, CXCR4 signaling events in rat and human neural progenitor cells (NPCs) were examined. Our results show that CXCR4 is expressed in abundance on rat and human NPCs. Moreover, SDF-1alpha induced increased NPCs levels of inositol 1,4,5-triphosphate, extracellular signal-regulated kinases 1/2, Akt, c-Jun N-terminal kinase, and intracellular calcium whereas it diminished cyclic adenosine monophosphate. Finally, SDF-1alpha can induce human NPC chemotaxis in vitro, suggesting that CXCR4 plays a functional role in NPC migration. Both T140, a CXCR4 antagonist, and pertussis toxin (PTX), an inactivator of G protein-coupled receptors, abrogated these events. Ultimately, this study suggested that SDF-1alpha can influence NPC function through CXCR4 and that CXCR4 is functional on NPC. |
| Databáze: | OpenAIRE |
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