Deletion of the C-terminal ESEV domain of NS1 does not affect the replication of a low-pathogenic avian influenza virus H7N1 in ducks and chickens
| Autor: | Thibaut Larcher, Romain Volmer, Daniel Marc, Guillaume Croville, Denis Soubieux, Thomas W Hoffmann, Sébastien M. Soubies, Pascale Quéré, Mireille Ledevin, Jean-Luc Guérin |
|---|---|
| Přispěvatelé: | Volmer, Romain, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Physiopathologie Animale et bioThérapie du muscle et du système nerveux (PAnTher), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Grant from the Direction Générale de l’Alimentation 366 (Fonds de Recherche Influenza Aviaires, project 08), Institut National de la Recherche Agronomique (INRA)-Université de Tours |
| Rok vydání: | 2012 |
| Předmět: |
modèle animal
animal structures [SDV]Life Sciences [q-bio] viruses Mutant poulet Virulence Chick Embryo Biology Viral Nonstructural Proteins medicine.disease_cause Virus Replication Virus virus grippal Cell Line intéraction hôte virus 03 medical and health sciences Immune system Virology medicine virus influenza aviaire Animals canard 030304 developmental biology Sequence Deletion 0303 health sciences 030306 microbiology virus diseases Influenza A virus subtype H5N1 Reverse genetics 3. Good health Ducks Viral replication génétique inverse Cell culture Influenza in Birds Influenza A Virus H7N1 Subtype modèle cellulaire Chickens |
| Zdroj: | Journal of General Virology 1 (94), 50-58. (2013) Journal of General Virology Journal of General Virology, Microbiology Society, 2013, 94 (1), pp.50-58. ⟨10.1099/vir.0.045153-0⟩ |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Highly pathogenic avian influenza (HPAI) H7N1 viruses caused a series of epizootics in Italy between 1999 and 2001. The emergence of these HPAI viruses coincided with the deletion of the six amino acids R225VESEV230at the C terminus of NS1. In order to assess how the truncation of NS1 affected virus replication, we used reverse genetics to generate a wild-type low-pathogenic avian influenza (LPAI) H7N1 virus with a 230aa NS1 (H7N1230) and a mutant virus with a truncated NS1 (H7N1224). The 6aa truncation had no impact on virus replication in duck or chicken cellsin vitro. The H7N1230and H7N1224viruses also replicated to similar levels and induced similar immune responses in ducks or chickens. No significant histological lesions were detected in infected ducks, regardless of the virus inoculated. However, in chickens, the H7N1230virus induced a more severe interstitial pneumonia than did the H7N1224virus. These findings indicate that the C-terminal extremity of NS1, including the PDZ-binding motif ESEV, is dispensable for efficient replication of an LPAI virus in ducks and chickens, even though it may increase virulence in chickens, as revealed by the intensity of the histological lesions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|