Natural Killer Receptor 1 Dampens the Development of Allergic Eosinophilic Airway Inflammation
| Autor: | Marilou Shagan, Avishai Shemesh, Ayelet Filiba, Itay Moshkovits, Shirin Elhaik Goldman, Ron N. Apte, Ariel Munitz, Elena Vronov, Ron Dagan, Danielle Karo-Atar, Angel Porgador, Yaffa Mizrachi Nebenzahl, D aniel Benharroch, Yevgeny Tsirulsky |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chemokine Physiology animal diseases lcsh:Medicine NK cells Immunoglobulin E Pathology and Laboratory Medicine Mice Immunologic Adjuvants 0302 clinical medicine Immune Physiology Cellular types Medicine and Health Sciences CCL17 Antigens Ly Public and Occupational Health lcsh:Science Lung Immune Response Innate Immune System Multidisciplinary biology medicine.diagnostic_test Chemotaxis Immune cells Animal Models respiratory system Vaccination and Immunization Cell Motility Cytokines White blood cells Tumor necrosis factor alpha medicine.symptom Chemokines Research Article Cell biology Blood cells Immunology Inflammation Mice Transgenic Mouse Models chemical and pharmacologic phenomena Research and Analysis Methods Peripheral blood mononuclear cell 03 medical and health sciences Th2 Cells Signs and Symptoms Model Organisms Diagnostic Medicine medicine Animals Natural Cytotoxicity Triggering Receptor 1 Macrophages lcsh:R Biology and Life Sciences Molecular Development biochemical phenomena metabolism and nutrition Asthma Eosinophils 030104 developmental biology Bronchoalveolar lavage Gene Expression Regulation Animal cells Immune System biology.protein bacteria lcsh:Q Preventive Medicine CCL24 030215 immunology Developmental Biology |
| Zdroj: | PLoS ONE, Vol 11, Iss 8, p e0160779 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The function of NCR1 was studied in a model of experimental asthma, classified as a type 1 hypersensitivity reaction, in mice. IgE levels were significantly increased in the serum of OVA immunized NCR1 deficient (NCR1gfp/gfp) mice in comparison to OVA immunized wild type (NCR1+/+) and adjuvant immunized mice. Histological analysis of OVA immunized NCR1gfp/gfp mice revealed no preservation of the lung structure and overwhelming peribronchial and perivascular granulocytes together with mononuclear cells infiltration. OVA immunized NCR+/+ mice demonstrated preserved lung structure and peribronchial and perivascular immune cell infiltration to a lower extent than that in NCR1gfp/gfp mice. Adjuvant immunized mice demonstrated lung structure preservation and no immune cell infiltration. OVA immunization caused an increase in PAS production independently of NCR1 presence. Bronchoalveolar lavage (BAL) revealed NCR1 dependent decreased percentages of eosinophils and increased percentages of lymphocytes and macrophages following OVA immunization. In the OVA immunized NCR1gfp/gfp mice the protein levels of eosinophils’ (CCL24) and Th2 CD4+ T-cells’ chemoattractants (CCL17, and CCL24) in the BAL are increased in comparison with OVA immunized NCR+/+ mice. In the presence of NCR1, OVA immunization caused an increase in NK cells numbers and decreased NCR1 ligand expression on CD11c+GR1+ cells and decreased NCR1 mRNA expression in the BAL. OVA immunization resulted in significantly increased IL-13, IL-4 and CCL17 mRNA expression in NCR1+/+ and NCR1gfp/gfp mice. IL-17 and TNFα expression increased only in OVA-immunized NCR1+/+mice. IL-6 mRNA increased only in OVA immunized NCR1gfp/gfp mice. Collectively, it is demonstrated that NCR1 dampens allergic eosinophilic airway inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|