Morphological and molecular analysis of the Nematostella vectensis cnidom
| Autor: | Mark-Oliver Diesner, Toshio Takahashi, Suat Özbek, Claudia Zenkert |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Hydra
ved/biology.organism_classification_rank.species Marine and Aquatic Sciences lcsh:Medicine Nematostella Genome Biochemistry Immunoenzyme Techniques Nematocyst Comparative Anatomy lcsh:Science Cells Cultured In Situ Hybridization Extracellular Matrix Proteins Multidisciplinary Starlet sea anemone Gene Expression Regulation Developmental Animal Models Lernaean Hydra Collagen Cnidocyte Research Article food.ingredient Blotting Western Molecular Sequence Data Morphogenesis Marine Biology Biology Evolution Molecular Cnidaria food Model Organisms Animals Amino Acid Sequence Model organism Evolutionary Biology Sequence Homology Amino Acid ved/biology Evolutionary Developmental Biology lcsh:R Proteins biology.organism_classification Molecular biology Evolutionary biology Earth Sciences lcsh:Q Zoology Developmental Biology |
| Zdroj: | PLoS ONE, Vol 6, Iss 7, p e22725 (2011) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The starlet sea anemone Nematostella vectensis is an emerging model organism for developmental and evolutionary biology. Due to the availability of genome data and its amenability to genetic manipulation Nematostella serves as a source for comparative molecular and phylogenetic studies. Despite this fact, the characterization of the nematocyst inventory and of nematocyst-specific genes is still fragmentary and sometimes misleading in this cnidarian species. Here, we present a thorough qualitative and quantitative analysis of nematocysts in Nematostella vectensis. In addition, we have cloned major nematocyst components, Nematostella minicollagens 1, 3 and 4, and show their expression patterns by in situ hybridization and immunocytochemistry using specific antibodies. Our data provides tools and insights for further studies on nematocyst morphogenesis in Nematostella and comparative evolution in cnidarians. |
| Databáze: | OpenAIRE |
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