Vascular integrin immunoreactivity is selectively lost on capillaries during rat focal cerebral ischemia and reperfusion
| Autor: | Martin Dichgans, Andreas Trinkl, Helge K. Martens, Gerhard F. Hamann, Jan Burk, Dorothe Burggraf |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Integrins medicine.medical_specialty Integrin alpha1 Integrin Ischemia Brain Edema Integrin alpha6 Brain Ischemia Laminin Internal medicine Animals Medicine Rats Wistar Molecular Biology Serum Albumin Cerebral Hemorrhage biology business.industry Cell adhesion molecule Integrin beta1 General Neuroscience Brain Endothelial Cells Anatomy Cerebral Arteries medicine.disease Immunohistochemistry Extravasation Capillaries Rats Endocrinology medicine.anatomical_structure Blood-Brain Barrier Reperfusion Injury biology.protein Basal lamina Neurology (clinical) Pericytes business Perfusion Developmental Biology |
| Zdroj: | Brain Research. 1189:189-197 |
| ISSN: | 0006-8993 |
| Popis: | The alpha1-integrin cell adhesion molecules, the principal endothelial receptors for basal lamina (BL) components disappear during transient ischemia. The current study investigated the localization of integrins, the time dependency and vessel size selectivity in the normal rat brain before and after 3 h of cerebral ischemia (I3) and reperfusion (R). Additionally we looked for a correlation to the amount of extravasation and hemorrhage. In the normal brain, there was a clear immunoreactivity for the alpha1, alpha6, and beta1 integrins on the endothelial perivascular cells. After I3 followed by variable reperfusion intervals of 0, 9, and 24 h (R0, R9 and R24; respectively), the number of vessels and staining intensity indicating immunoreactivity in the ischemic area were compared with the contralateral side. The number of the beta1-immunoreactive capillaries was steadily decreasing with the reperfusion time: -12+/-5%, -15+/-7% and -43+/-8% at I3R0, I3R9 and I3R24 (all p |
| Databáze: | OpenAIRE |
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