The Stimulation of T Cells with Anti-CD2 Monoclonal Antibodies Facilitates the Induction of Polyclonal B-Cell Responses but does not Enhance the Activation of Antigen-Specific B Cells
| Autor: | R. Trifiletti, Gabriel Virella, M. LaVIA, Barbara Hyman, W. Knapp |
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| Rok vydání: | 1990 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Interleukin 2 Erythrocytes medicine.drug_class T-Lymphocytes Immunology CD2 Antigens Lymphocyte Activation Monoclonal antibody Epitope Antigen Antibody Specificity medicine Humans Receptors Immunologic Cells Cultured B cell B-Lymphocytes Membrane Glycoproteins biology Antibodies Monoclonal General Medicine T lymphocyte CD58 Antigens Molecular biology medicine.anatomical_structure Immunoglobulin M Pokeweed Mitogens Polyclonal antibodies Immunoglobulin G Antigens Surface Hemocyanins biology.protein Interleukin-2 Antibody medicine.drug |
| Zdroj: | Scandinavian Journal of Immunology. 31:25-31 |
| ISSN: | 1365-3083 0300-9475 |
| DOI: | 10.1111/j.1365-3083.1990.tb02739.x |
| Popis: | In this report we compare the effect of stimulation of peripheral mononuclear cells (PBMC) by using two monoclonal antibodies (MoAb) directed against the CD2 receptor on T cells or by using autologous erythrocytes (E) which express on their surface lymphocyte function-associated antigen 3 (LFA3), a natural ligand for CD2. The addition of autologous erythrocytes to pokeweed mitogen (PWM)-stimulated PBMC results in the enhancement of polyclonal immunoglobulin synthesis and of antigen-specific B-cell responses. Because B cells lack the CD2 molecule, it can be concluded that their enhanced activity is a consequence of the delivery of activating signals by activated T lymphocytes. When PBMC cultures were stimulated with a pair of anti-CD2 MoAb (Leu5b and VIT13) we were able to induce polyclonal immunoglobulin synthesis, particularly IgM, in cultures supplemented with interleukin 2(IL-2). Specific responses to tetanus toxoid (TT) and keyhole limpet haemocyanin (KLH) were also enhanced by the addition of autologous E to PWM-stimulated PBMC. Significant anti-TT responses were observed in cultures stimulated with E + TT + IL-2. In contrast, stimulation of PBMC with VIT13 + Leu5b + IL-2 + antigen was not effective in inducing anti-TT antibody and only weakly effective in inducing anti-KLH antibodies. Replacing Leu5b by anti-CD3 had no effect on the induction of specific antibody responses; in contrast, replacement of Leu5b by E enhanced anti-TT antibody production while the effect on polyclonal production of IgM was minimal. Therefore, it appears that the signal delivered by the association of CD2 with LFA3 is a better potentiating signal for specific B-cell responses than the signal delivered by pairs of MoAb to different epitopes of CD2 or to CD2 and CD3 epitopes. |
| Databáze: | OpenAIRE |
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