Role of the endocannabinoid system in the mechanisms involved in the LPS-induced preterm labor
| Autor: | Ana Maria Franchi, Julieta Aisemberg, Maria Victoria Bariani, Ana Paula Domínguez Rubio, Maximiliano Cella, Juliana Burdet |
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| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Embryology Cannabinoid receptor Time Factors Lipopolysaccharide medicine.medical_treatment purl.org/becyt/ford/1.7 [https] Dinoprost purl.org/becyt/ford/1 [https] Receptor Cannabinoid CB2 chemistry.chemical_compound Endocrinology Receptor Cannabinoid CB1 Fatty acid amide hydrolase Pregnancy Receptor Progesterone Mice Inbred BALB C Otras Ciencias Naturales y Exactas Obstetrics and Gynecology Anandamide Endocannabinoid system Endocannabinoides lipids (amino acids peptides and proteins) Female CIENCIAS NATURALES Y EXACTAS medicine.medical_specialty Lps Polyunsaturated Alkamides Gestational Age Arachidonic Acids Biology Parto Prematuro Amidohydrolases Obstetric Labor Premature Internal medicine medicine Phospholipase D Animals Cannabinoid Receptor Antagonists Uterus Cell Biology Disease Models Animal Reproductive Medicine chemistry Gene Expression Regulation Cannabinoid receptor antagonist Cannabinoid Endocannabinoids |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1741-7899 |
| Popis: | Prematurity is the leading cause of perinatal morbidity and mortality worldwide. There is a strong causal relationship between infection and preterm births. Intrauterine infection elicits an immune response involving the release of inflammatory mediators like cytokines and prostaglandins (PG) that trigger uterine contractions and parturition events. Anandamide (AEA) is an endogenous ligand for the cannabinoid receptors CB1 and CB2. Similarly to PG, endocannabinoids are implicated in different aspects of reproduction, such as maintenance of pregnancy and parturition. Little is known about the involvement of endocannabinoids on the onset of labor in an infectious milieu. Here, using a mouse model of preterm labor induced by lipopolysaccharide (LPS), we explored changes on the expression of components of endocannabinoid system (ECS). We have also determined whether AEA and CB antagonists alter PG production that induces labor. We observed an increase in uterine N-acylphosphatidylethanolamine-specific phospholipase D expression (NAPE-PLD, the enzyme that synthesizes AEA) upon LPS treatment. Activity of catabolic enzyme fatty acid amide hydrolase (FAAH) did not change significantly. In addition, we also found that LPS modulated uterine cannabinoid receptors expression by downregulating Cb2 mRNA levels and upregulating CB1 protein expression. Furthermore, LPS and AEA induced PGF2a augmentation, and this was reversed by antagonizing CB1 receptor. Collectively, our results suggest that ECS may be involved in the mechanism by which infection causes preterm birth. Fil: Bariani, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Domínguez Rubio, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Cella, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Burdet, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Franchi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Aisemberg, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina |
| Databáze: | OpenAIRE |
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