Changes in Global Transcriptional Profiling of Women Following Obesity Surgery Bypass
| Autor: | Wilson Salgado Junior, Julio Sérgio Marchini, Cristiana Cortes-Oliveira, Natália Yumi Noronha, Wilson Araújo da Silva Júnior, Ana Julia Machry, Carla Barbosa Nonino, Bruno Affonso Parente de Oliveira, Carolina Ferreira Nicoletti, Vitor Caressato Pinhanelli, Marcela Augusta de Souza Pinhel, Dorotéia Rossi Silva Souza |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Pathology medicine.medical_specialty Endocrinology Diabetes and Metabolism Cellular differentiation Gastric Bypass Bariatric Surgery Gene Expression Transcriptome 03 medical and health sciences Weight Loss Gene expression Humans Medicine HSF1 Gene Nutrition and Dietetics biology business.industry Microarray analysis techniques Gene Expression Profiling EXPRESSÃO GÊNICA Middle Aged Microarray Analysis Obesity Morbid Gene expression profiling 030104 developmental biology Case-Control Studies Leukocytes Mononuclear Cancer research biology.protein Female Surgery Mitogen-Activated Protein Kinases business CREB1 |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1708-0428 0960-8923 |
| Popis: | Differential gene expression in peripheral blood mononuclear cells (PBMCs) after Roux-en-Y gastric bypass (RYGB) is poorly characterized. Markers of these processes may provide a deeper understanding of the mechanisms that underlie these events. The main goal of this study was to identify changes in PBMC gene expression in women with obesity before and 6 months after RYGB-induced weight loss. The ribonucleic acid (RNA) of PBMCs from 13 obese women was analyzed before and 6 months after RYGB; the RNA of PBMCs from nine healthy women served as control. The gene expression levels were determined by microarray analysis. Significant differences in gene expression were validated by real-time quantitative polymerase chain reaction (RT-qPCR). Microarray analysis for comparison of the pre- and postoperative periods showed that 1366 genes were differentially expressed genes (DEGs). The main pathways were related to gene transcription; lipid, energy, and glycide metabolism; inflammatory and immunological response; cell differentiation; oxidative stress regulation; response to endogenous and exogenous stimuli; substrate oxidation; mTOR signaling pathway; interferon signaling; mitogen-activated protein kinases (MAPK), cAMP response element binding protein (CREB1), heat shock factor 1 (HSF1), and sterol regulatory element binding protein 1c (SREBP-1c) gene expression; adipocyte differentiation; and methylation. Six months after bariatric surgery and significant weight loss, many molecular pathways involved in obesity and metabolic diseases change. These findings are an important tool to identify potential targets for therapeutic intervention and clinical practice of nutritional genomics in obesity. |
| Databáze: | OpenAIRE |
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