PRAK-03202: A triple antigen virus-like particle vaccine candidate against SARS CoV-2
| Autor: | Jeny Paliwal, Prabuddha Kumar Kundu, Dilip Kumar, Sriganesh Kalyanaraman, Sourav Singha Roy, Nupur Mehrotra Arora, Ruchir Rastogi, Manish Saini, Biswa Pratim, Saumyabrata Mazumder, Abyson Joseph, Avinash Undale, Swaroop Potdar, Kajal Arora, D. Kumar, Apoorva Srivastava, Sarthi Bansal, Kshipra Jain, Satabdi Rautray, Manglesh Kumar Singh, Vidya Bhushan Arya, R. S. Gupta, Sudhir Kumar Singh, Aditi Gupta, Bhupesh Mali, Deepak Parashar, Aelia Naqvi, Abhishek Mukherjee |
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| Rok vydání: | 2021 |
| Předmět: |
Science (General)
Lymphocyte proliferation medicine.disease_cause Epitope Q1-390 Immune system Virus-like particle Antigen Interferon Electron microscopy medicine Humoral immune response Coronavirus H1-99 Multidisciplinary biology Convalescent COVID-19 patients Virus like particle Virology Social sciences (General) biology.protein Antibody Corrigendum Vaccine Research Article medicine.drug |
| Zdroj: | Heliyon Heliyon, Vol 7, Iss 10, Pp e08124-(2021) |
| ISSN: | 2405-8440 |
| Popis: | The rapid development of safe and effective vaccines against severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) is a necessary response to coronavirus outbreak. Here, we developed PRAK-03202, the world's first triple antigen virus-like particle vaccine candidate, by cloning and transforming SARS-CoV-2 gene segments into a highly characterized S. cerevisiae-based D-Crypt™ platform, which induced SARS CoV-2 specific neutralizing antibodies in BALB/c mice. Immunization using three different doses of PRAK-03202 induced an antigen-specific (spike, envelope, and membrane proteins) humoral response and neutralizing potential. Peripheral blood mononuclear cells from convalescent patients showed lymphocyte proliferation and elevated interferon levels suggestive of epitope conservation and induction of T helper 1-biased cellular immune response when exposed to PRAK-03202. These data support further clinical development and testing of PRAK-03202 for use in humans. Virus like particle; Vaccine; Humoral immune response; Convalescent COVID-19 patients; Electron microscopy. |
| Databáze: | OpenAIRE |
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