n-6 High Fat Diet Induces Gut Microbiome Dysbiosis and Colonic Inflammation
Autor: | Christopher Smith, Justin M. Snider, Thomas Doetschman, Micah G. Donovan, Donato F. Romagnolo, Sherry H.-H. Chow, Spencer N. Wren, Nick Greenfield, Andreas J. Papoutsis, Ornella I. Selmin, Ashley J. Snider, Sabine Hazan |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
QH301-705.5 Offspring Firmicutes omega-6 Physiology fatty acids Article Catalysis Proinflammatory cytokine Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine medicine Weaning Microbiome Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy chemistry.chemical_classification biology colon Organic Chemistry Lachnospiraceae General Medicine dysbiosis medicine.disease biology.organism_classification Computer Science Applications Chemistry 030104 developmental biology chemistry inflammation 030220 oncology & carcinogenesis gut Dysbiosis Polyunsaturated fatty acid |
Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 13 International Journal of Molecular Sciences, Vol 22, Iss 6919, p 6919 (2021) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms22136919 |
Popis: | Background: Concerns are emerging that a high-fat diet rich in n-6 PUFA (n-6HFD) may alter gut microbiome and increase the risk of intestinal disorders. Research is needed to model the relationships between consumption of an n-6HFD starting at weaning and development of gut dysbiosis and colonic inflammation in adulthood. We used a C57BL/6J mouse model to compare the effects of exposure to a typical American Western diet (WD) providing 58.4%, 27.8%, and 13.7% energy (%E) from carbohydrates, fat, and protein, respectively, with those of an isocaloric and isoproteic soybean oil-rich n-6HFD providing 50%E and 35.9%E from total fat and carbohydrates, respectively on gut inflammation and microbiome profile. Methods: At weaning, male offspring were assigned to either the WD or n-6HFD through 10–16 weeks of age. The WD included fat exclusively from palm oil whereas the n-6HFD contained fat exclusively from soybean oil. We recorded changes in body weight, cyclooxygenase-2 (COX-2) expression, colon histopathology, and gut microbiome profile. Results: Compared to the WD, the n-6HFD increased plasma levels of n-6 fatty acids colonic expression of COX-2 and the number of colonic inflammatory and hyperplastic lesions. At 16 weeks of age, the n-6HFD caused a marked reduction in the gut presence of Firmicutes, Clostridia, and Lachnospiraceae, and induced growth of Bacteroidetes and Deferribacteraceae. At the species level, the n-6HFD sustains the gut growth of proinflammatory Mucispirillum schaedleri and Lactobacillus murinus. Conclusions: An n-6HFD consumed from weaning to adulthood induces a shift in gut bacterial profile associated with colonic inflammation. |
Databáze: | OpenAIRE |
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