Amelioration of allergic airway inflammation in mice by regulatory IL-35 through dampening inflammatory dendritic cells
| Autor: | Chun-Kwok Wong, Jie Dong, Christopher Wai-Kei Lam, Zhe Cai, Delong Jiao, Minjie Chu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Ovalbumin
medicine.medical_treatment Immunology Risk Assessment Allergic sensitization Mice Random Allocation Th2 Cells Reference Values Respiratory Hypersensitivity medicine Animals Humans Immunology and Allergy Cells Cultured Mice Inbred BALB C Lung medicine.diagnostic_test biology business.industry Dendritic Cells respiratory system Eosinophil Interleukin-12 Asthma Disease Models Animal Treatment Outcome medicine.anatomical_structure Bronchoalveolar lavage Cytokine Integrin alpha M Interleukin 35 biology.protein Cytokines Immunization Lymph business Injections Intraperitoneal |
| Zdroj: | Allergy. 70:921-932 |
| ISSN: | 0105-4538 |
| Popis: | Background IL-35, a new member of the IL-12 family, is an inhibitory cytokine produced by regulatory T and B lymphocytes that play a suppressive role in the inflammatory diseases. This study focuses on the cellular mechanism regulating the anti-inflammatory activity of IL-35 in asthmatic mice. Methods Ovalbumin-induced asthmatic and humanized asthmatic mice were adopted to evaluate the in vivo anti-inflammatory activities of IL-35. For monitoring the airway, Penh value (% baseline) was measured using a whole-body plethysmograph. Results In this study using ovalbumin-induced asthmatic mice, we observed that intraperitoneal injection of IL-35 during the allergen sensitization stage was more efficient than administration in the challenge stage for the amelioration of airway hyper-responsiveness. This was reflected by the significantly reduced concentration of asthma-related Th2 cytokines IL-5 and IL-13, as well as eosinophil counts in bronchoalveolar lavage fluid (all P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text