The Correlation between the Determination of Vaginal Micro-Ecological Composition and the Outcome of HPV Infection by High-Throughput Metagene Sequencing Information Technology on the Illumina Platform
| Autor: | Leilei Gao, Fei Xu, Jinwei Liu, Weiye Cheng |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology medicine.disease_cause DNA sequencing lcsh:Infectious and parasitic diseases Correlation 03 medical and health sciences 0302 clinical medicine Lactobacillus medicine Gardnerella vaginalis Humans lcsh:RC109-216 030212 general & internal medicine Papillomaviridae High-Throughput Metagene Sequencing Information Technology gene sequencing biology business.industry Vaginal flora lcsh:Public aspects of medicine Papillomavirus Infections vaginal flora Public Health Environmental and Occupational Health HPV infection High-Throughput Nucleotide Sequencing lcsh:RA1-1270 General Medicine Mycoplasma vagina microecology medicine.disease biology.organism_classification female genital diseases and pregnancy complications Infectious Diseases Immunology Vagina Female business Information Technology Ureaplasma urealyticum |
| Zdroj: | Journal of Infection and Public Health, Vol 13, Iss 12, Pp 1961-1966 (2020) |
| ISSN: | 1876-035X |
| Popis: | Objective To study the correlation between vaginal micro-ecological composition and the outcome of human papillomavirus (HPV) infection by High-Throughput Metagene Sequencing Information Technology on the Illumina Platform, and to improve the efficiency of clinical infection detection. Methods: With the aid of Illumina high-throughput sequencing platform and sequence research method, the composition and diversity of vaginal microorganisms in high-risk HPV (HR-HPV) infected women and healthy women were analyzed. The differences in vaginal flora of HR-HPV infected women and healthy women were compared to explore the correlation between HR-HPV infection and vaginal flora. Results: The structure of vaginal flora in healthy women was relatively single, with Lactobacillus as the dominant genus, accounting for more than 80%. The structure of vaginal flora in women infected with HR-HPV was significantly different from that in non-infected women. The former had a significantly increased species diversity, which was mainly manifested by a decrease in Lactobacillus and an increase in Gardnerella vaginalis. Mycoplasma and Ureaplasma urealyticum might play a synergistic role in the initial stage of cervical lesions caused by HR-HPV infection. Conclusion: The prevention and treatment of mycoplasma and Ureaplasma urealyticum should be valued clinically to prevent the occurrence of HR-HPV infection and cervical lesions. |
| Databáze: | OpenAIRE |
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