Effects of high versus standard essential amino acid intakes on whole-body protein turnover and mixed muscle protein synthesis during energy deficit: A randomized, crossover study
| Autor: | Lee M. Margolis, John W. Carbone, Nancy E. Murphy, Jess A. Gwin, Marques A. Wilson, Emily E. Howard, David D. Church, Robert R. Wolfe, Arny A. Ferrando, Christopher T. Carrigan, Adrienne Hatch-McChesney, Stefan M. Pasiakos |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine Anabolism Energy balance Muscle Proteins 030209 endocrinology & metabolism Critical Care and Intensive Care Medicine Young Adult 03 medical and health sciences 0302 clinical medicine Animal science Double-Blind Method Humans Medicine Ingestion Energy deficit Exercise Essential amino acid Caloric Restriction chemistry.chemical_classification Cross-Over Studies 030109 nutrition & dietetics Nutrition and Dietetics business.industry Protein turnover Postprandial Period Crossover study Postprandial chemistry Protein Biosynthesis Proteolysis Amino Acids Essential Energy Intake business |
| Zdroj: | Clinical Nutrition. 40:767-777 |
| ISSN: | 0261-5614 0337-2928 |
| Popis: | Summary Background & aims Consuming 0.10–0.14 g essential amino acids (EAA)/kg/dose (0.25–0.30 g protein/kg/dose) maximally stimulates muscle protein synthesis (MPS) during energy balance. Whether consuming EAA beyond that amount enhances MPS and whole-body anabolism following energy deficit is unknown. The aims of this study were to determine the effects of standard and high EAA ingestion on mixed MPS and whole-body protein turnover following energy deficit. Design Nineteen males (mean ± SD; 23 ± 5 y; 25.4 ± 2.7 kg/m2) completed a randomized, double-blind crossover study consisting of two, 5-d energy deficits (−30 ± 4% of total energy requirements), separated by 14-d. Following each energy deficit, mixed MPS and whole-body protein synthesis (PS), breakdown (PB), and net balance (NET) were determined at rest and post-resistance exercise (RE) using primed, constant L-[2H5]-phenylalanine and L-[2H2]-tyrosine infusions. Beverages providing standard (0.1 g/kg, 7.87 ± 0.87 g) or high (0.3 g/kg, 23.5 ± 2.54 g) EAA were consumed post-RE. Circulating EAA were measured. Results Postabsorptive mixed MPS (%/h) at rest was not different (P = 0.67) between treatments. Independent of EAA, postprandial mixed MPS at rest (standard EAA, 0.055 ± 0.01; high EAA, 0.061 ± 0.02) and post-RE (standard EAA, 0.055 ± 0.01; high EAA, 0.065 ± 0.02) were greater than postabsorptive mixed MPS at rest (P = 0.02 and P = 0.01, respectively). Change in (Δ postabsorptive) whole-body (g/180 min) PS and PB was greater for high than standard EAA [mean treatment difference (95% CI), 3.4 (2.3, 4.4); P = 0.001 and −15.6 (−17.8, −13.5); P = 0.001, respectively]. NET was more positive for high than standard EAA [19.0 (17.3, 20.7); P = 0.001]. EAA concentrations were greater in high than standard EAA (P = 0.001). Conclusions These data demonstrate that high compared to standard EAA ingestion enhances whole-body protein status during underfeeding. However, the effects of consuming high and standard EAA on mixed MPS are the same during energy deficit. Clinical trial registry NCT03372928 , https://clinicaltrials.gov . |
| Databáze: | OpenAIRE |
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