Antifungi Effect of Keghenyat Leaves Fraction (Acmella uliginosa) In Vitro Study
| Autor: | Joko Marwoto, Ziske Maritska, Agustina Septi Hijir, Triwani Triwani, Evi Lusiana, Ayeshah Augusta Rosdah, Sri Nita, Nia Savitri Tamzil, Nita Parisa, Salni Salni, Mgs Irsan Saleh |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
biology
Traditional medicine lcsh:RM1-950 Fraction (chemistry) Fungus Acmella uliginosa biology.organism_classification Terpenoid Yeast lcsh:RC346-429 Minimum inhibitory concentration Nystatin lcsh:Therapeutics. Pharmacology medicine Candida albicans lcsh:Neurology. Diseases of the nervous system medicine.drug |
| Zdroj: | Bioscientia Medicina: Journal of Biomedicine and Translational Research, Vol 1, Iss 1, Pp 1-7 (2017) BASE-Bielefeld Academic Search Engine |
| ISSN: | 2598-0580 |
| Popis: | Introduction Candida is a yeast fungus classes most commonly found in the oral cavity, gastrointestinal tract, reproductive tract and skin, especially the species Candida albicans. Alternative medicines such as herbs are easily available and affordable by the community, such as keghenyat leaves (Acmella uliginosa). Aim of StudyTo determine the activity of Keghenyat leaves fraction (Acmella uliginosa) compared to Nystatin against Candida albicans in vitro. MethodsIn vitro study was held in Laboratory of Genetics and Biotechnology Department of Biology, Faculty of Science and Medical Faculty, Sriwijaya University in December 2015 and January 2016. Research stages: extraction, fractionation, sensitivity test using Nystatin and Candida albicans, fractions antifungal activity test, the determination of minimum inhibitory concentration (MIC), bioautografi test and determination of compound classes. One final stages of testing the active fraction Keghenyat leaves (Acmella uliginosa) with Nystatin. Data were analyzed using ANOVA and Post Hoc Duncan test and linear regression using SPSS 20. ResultsHexane faction of Keghenyat leaves (Acmella uliginosa) is active against the fungus Candida albicans, has a Minimum Inhibitory Concentration (MIC) of 0.625% (6250μl) against the fungus Candida albicans antifungal contain active compounds that terpenoids and flavonoids, amounting to 84.68 mg / ml equivalent to 1 mg / ml Nystatin against the fungus Candida albicans. ConclusionThere is a significance different MIC between hexane fraction of Keghenyat leaves (Acmella uliginosa) and Nystatin, Nystatin have more high activity. |
| Databáze: | OpenAIRE |
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