ALK and EGFR expression by immunohistochemistry are associated with Merkel cell polyomavirus status in Merkel cell carcinoma
| Autor: | Virve Koljonen, Tuukka Veija, Tom Böhling, Mia Kero |
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| Přispěvatelé: | Department of Pathology, Medicum, University of Helsinki, HUSLAB, Clinicum, Plastiikkakirurgian yksikkö, Department of Surgery, Tom Böhling / Principal Investigator |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Skin Neoplasms Merkel cell polyomavirus Gene mutation Metastasis 0302 clinical medicine Merkel cell carcinoma hemic and lymphatic diseases antibody Medicine Anaplastic Lymphoma Kinase Tissue microarray biology EZH2 food and beverages General Medicine Middle Aged 3. Good health ErbB Receptors 030220 oncology & carcinogenesis immunohistochemistry Immunohistochemistry Female Oncovirus circulatory and respiratory physiology Histology Pathology and Forensic Medicine 03 medical and health sciences Biomarkers Tumor Humans Enhancer of Zeste Homolog 2 Protein protein expression Aged Polyomavirus Infections business.industry medicine.disease biology.organism_classification sensitivity mutations infection Carcinoma Merkel Cell Tumor Virus Infections 030104 developmental biology Cancer research 3111 Biomedicine business |
| Popis: | Aims Merkel cell carcinoma, a rare cutaneous neuroendocrine tumour of the skin, can be categorised into two groups according to Merkel cell polyomavirus (MCV) presence. MCV-negative tumours are more aggressive and frequently associated with gene mutations. Some of the genes are potential therapeutic targets. We have previously reported EGFR mutations in six of 27 MCC tumours and overexpression of ALK and EZH2 at mRNA level in MCC tumours. In this study, we sought to determine expression of ALK, EGFR and EZH2 in MCC samples and assess their correlation to MCV status and clinical parameters. Methods and results Tissue microarrays were utilised and stained with primary antibodies. Staining data were statistically compared to patient sex, tumour location and development of metastasis and MCC-specific death; 112 tumours and their corresponding patient data were included. We found strong expression of ALK in 51% and strong expression of EZH2 in 76% of the tumours. There was evident correlation of ALK expression with MCV-positivity. Expression of EGFR was infrequent, presenting only in seven MCV-negative tumours. None of the proteins associated with development of metastasis or MCC specific death. Conclusions ALK and EZH2 expression are frequent in MCC and ALK expression correlates to MCV positivity. EGFR positive tumours might respond to EGFR inhibiting treatment. |
| Databáze: | OpenAIRE |
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