Effects of the Antioxidative -Blocker Celiprolol on Endothelial Progenitor Cells in Hypertensive Rats
| Autor: | Takahiro Ueno, Koichi Matsumoto, Noboru Fukuda, Naohiko Kobayashi, Ying Han, Taro Matsumoto, En-Hui Yao, Mayumi Katakawa, Chii Yamamoto |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Senescence medicine.medical_specialty Renal cortex Adrenergic beta-Antagonists medicine.disease_cause Rats Inbred WKY Antioxidants Cell Movement Rats Inbred SHR Internal medicine Internal Medicine medicine TBARS Animals RNA Messenger Progenitor cell Cells Cultured Celiprolol business.industry Stem Cells Endothelial Cells NADPH Oxidases Atenolol Rats Endothelial stem cell Oxidative Stress Endocrinology medicine.anatomical_structure Hypertension embryonic structures cardiovascular system business Oxidative stress circulatory and respiratory physiology medicine.drug |
| Zdroj: | American Journal of Hypertension. 21:1062-1068 |
| ISSN: | 1941-7225 0895-7061 |
| DOI: | 10.1038/ajh.2008.233 |
| Popis: | BACKGROUND Endothelial progenitor cells (EPCs) derived from bone marrow migrate to areas of endothelial damage and repair them. EPC function is impaired by oxidative stress. We examined the effects of an antioxidative beta1-adrenoceptor blocker on the number and function of EPCs in hypertensive rats. METHODS Spontaneously hypertensive rats (SHRs) and Wistar-Kyoto (WKY) rats were fed diets loaded with high salt. The SHRs were treated with celiprolol or atenolol for 2 weeks. Peripheral blood mononuclear cells (MNCs) were separated, subjected to flow cytometric analysis to determine the number of circulating EPCs, and cultured to quantify EPC colony formation. EPC migration was evaluated in migration assay chambers. EPC senescence was evaluated using beta-galactosidase assay. Oxidative stress of EPCs was evaluated using thiobarbituric acid-reactive substance (TBARS) assay. The expression of nicotinamine adenine dinucleotide phosphate (NAD(P)H) oxidase component mRNAs in the renal cortex, aorta, and heart were evaluated by real-time PCR. RESULTS The number, colony formation, and migration of EPCs in SHRs were significantly lower than those in WKY rats. TBARS scores in EPCs from SHRs were significantly higher than those from WKY rats. Celiprolol increased the number of circulating EPCs and stimulated EPC colony formation and migration, while decreasing EPC senescence. Celiprolol inhibited oxidation in EPCs from SHRs, and decreased the expression of NAD(P)H oxidase component mRNAs in the renal cortex, aorta, and heart. CONCLUSION EPCs are impaired in SHRs in response to oxidative stress. Celiprolol decreases oxidative stress in hypertension in vivo and improves EPC numbers and function. It appears, therefore, that celiprolol may exert beneficial cardiovascular effects through its antioxidative properties. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|