Photoinhibition of Streptococcus mutans Biofilm-Induced Lesions in Human Dentin by Violet-Blue Light
| Autor: | Andrea Ferreira Zandona, Frank Lippert, Grace F. Gomez, Masatoshi Ando, George J. Eckert, Richard L. Gregory |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Sucrose
Photoinhibition 030303 biophysics Tryptic soy broth Article Streptococcus mutans 03 medical and health sciences chemistry.chemical_compound Microtiter plate 0302 clinical medicine violet-blue light Food science General Dentistry Colony-forming unit 0303 health sciences biology Chemistry Biofilm food and beverages 030206 dentistry biochemical phenomena metabolism and nutrition biology.organism_classification equipment and supplies Fluorescence dental caries phototherapy |
| Zdroj: | Dentistry Journal Volume 7 Issue 4 |
| ISSN: | 2304-6767 |
| DOI: | 10.3390/dj7040113 |
| Popis: | This in vitro study determined the effectiveness of violet-blue light on Streptococcus mutans (UA159) biofilm induced dentinal lesions. Biofilm was formed on human dentin specimens in a 96-well microtiter plate and incubated for 13 h in the presence of tryptic soy broth (TSB) or TSB supplemented with 1% sucrose (TSBS). Violet-blue light (405 nm) from quantitative light-induced fluorescence (QLFTM) was used to irradiate the biofilm. Supernatant liquid was removed, and the biofilm was irradiated continuously with QLF for 5 min twice daily with an interval of 6 h for 5 d, except with one treatment on the final day. Colony forming units (CFU) of the treated biofilm, changes in fluorescence (∆F QLF-Digital BiluminatorTM), lesion depth (L), and integrated mineral loss (∆Z both transverse microradiography) were quantified at the end of the fifth day. Statistical analysis used analysis of variance (ANOVA), testing at a 5% significance level. In the violet-blue light irradiated groups, there was a significant reduction (p < 0.05) of bacterial viability (CFU) of S. mutans with TSB and TSBS. Violet-blue light irradiation resulted in the reduction of ∆F and L of the dentinal surface with TSBS. These results indicate that violet-blue light has the capacity to reduce S. mutans cell numbers. |
| Databáze: | OpenAIRE |
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