Effect of pterostilbene on development, equatorial lipid accumulation and reactive oxygen species production of in vitro‐produced bovine embryos
| Autor: | S. Romo, Sandra Pérez‐Reynozo, Froylan Sosa, M. E. Kjelland, José Fernando De La Torre-Sánchez, Horacio Alvarez-Gallardo, David Urbán-Duarte |
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| Rok vydání: | 2020 |
| Předmět: |
Pterostilbene
Embryonic Development Fertilization in Vitro Cryopreservation Embryo Culture Techniques Andrology chemistry.chemical_compound Endocrinology Stilbenes medicine Animals Blastocyst chemistry.chemical_classification Reactive oxygen species Phytoalexin Embryo Embryo Transfer Lipid Metabolism In vitro medicine.anatomical_structure chemistry embryonic structures Lipogenesis Cattle Animal Science and Zoology Reactive Oxygen Species Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 55:1490-1500 |
| ISSN: | 1439-0531 0936-6768 |
| Popis: | The pterostilbene (PT) molecule is a phytoalexin with a reducing effect on reactive oxygen species (ROS) and with a capacity to block lipogenesis. However, the potential reducing effects of PT on equatorial lipid accumulation and ROS have not yet been elucidated for in vitro-derived bovine embryos. The present study evaluated the effects of concentrations of 3, 1, 0.33, 0.11 μM PT, and a vehicle group on the percentage of cleaved embryos, embryos with more than 6 cells, percentage of blastocyst on Day 7 and 8, percentage of transferable embryos on Day 7, the cell count and relative concentration of lipids. In the second experiment, the effects of 0.33 μM PT and a vehicle group within two different O2 environments (5% and 20%) were evaluated for ROS generation and the percentage of Day 8 blastocysts. In the first experiment, no significant differences were found between the treatments with PT and the vehicle group (p > .05) concerning the percentage of cleaved embryos and embryos with more than 6 cells. Lipid reduction was observed in the groups treated with PT versus the vehicle group (p .05). In the second experiment, the O2 concentration did not significantly affect ROS generation (p > .05); however, the groups treated with PT (0.33 μM) had a reduction in ROS (p |
| Databáze: | OpenAIRE |
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