White spot syndrome virus VP12 interacts with adenine nucleotide translocase of Litopenaeus vannamei
| Autor: | Jie Huang, Qing-hui Liu, Chen Li, Fang-fang Ma, Zhi-guang Chou, Guangkuo Guan |
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| Rok vydání: | 2013 |
| Předmět: |
White spot syndrome
Amino Acid Motifs Blotting Western Molecular Sequence Data Litopenaeus Enzyme-Linked Immunosorbent Assay Mitochondrion Polymerase Chain Reaction Mass Spectrometry Host-Parasite Interactions Viral Proteins White spot syndrome virus 1 Penaeidae Animals Amino Acid Sequence Ecology Evolution Behavior and Systematics Cellular localization Messenger RNA biology Base Sequence biology.organism_classification Molecular biology Recombinant Proteins Shrimp Blot Cytoplasm Mitochondrial ADP ATP Translocases |
| Zdroj: | Journal of invertebrate pathology. 118 |
| ISSN: | 1096-0805 |
| Popis: | White spot syndrome virus VP12 contains cell attachment motif RGD which is considered to be critical for host cell binding. Until now, the function of this protein remains undefined. In this study, we explored the interaction of VP12 with host cells. A new shrimp protein (adenine nucleotide translocase of Litopenaeus vannamei, LvANT) is selected by far-western overlay assay. Tissue distribution of adenine nucleotide translocase mRNA showed that it was commonly spread in all the tissues detected. Cellular localization of LvANT in shrimp hemocytes showed that it was primarily located in the cytoplasm of hemocytes and colocalized with mitochondria. ELISA and far-western blot assay confirmed that VP12 interacted with LvANT. In vivo neutralization assay showed that anti-LvANT antibody can significantly reduce the mortality of shrimp challenged by WSSV at 48h post-treatment. Our results collectively showed that VP12 is involved in host cell binding via interaction with adenine nucleotide translocase. |
| Databáze: | OpenAIRE |
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