Dietary hydrogenated vegetable fat exacerbates the activation of kynurenine pathway caused by peripheral lipopolysaccharide immune challenge in aged mice
Autor: | Franciele Donato, Robson Luiz Puntel, Lucian Del Fabbro, André Tiago Rossito Goes, Marcelo Gomes de Gomes, Silvana Peterini Boeira, Cristiano Ricardo Jesse, Carlos Borges Filho, Leandro Cattelan Souza |
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Rok vydání: | 2018 |
Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Kynurenine pathway genetic structures Lipopolysaccharide Prefrontal Cortex Inflammation Kynurenic Acid Toxicology Hippocampus Proinflammatory cytokine Interferon-gamma Mice 03 medical and health sciences chemistry.chemical_compound Kynurenine 3-Monooxygenase 0302 clinical medicine Kynurenic acid Internal medicine Vegetables medicine Animals Indoleamine-Pyrrole 2 3 -Dioxygenase Kynurenine Transaminases Sickness behavior Tumor Necrosis Factor-alpha business.industry Tryptophan General Medicine Dietary Fats Corpus Striatum 030227 psychiatry Mice Inbred C57BL Endocrinology chemistry medicine.symptom Corticosterone business Locomotion 030217 neurology & neurosurgery Quinolinic acid |
Zdroj: | Chemico-Biological Interactions. 293:28-37 |
ISSN: | 0009-2797 |
Popis: | Sickness behavior is a normal immune response of body to fight infection, accompanied by endocrine and behavioral alterations. Lipopolysaccharide (LPS) causes sickness behavior in rodents through the increase of proinflammatory cytokines, generating peripheral inflammation and thus overactivation of kynurenine pathway (KP). In the present study we investigated the effects of dietary hydrogenated vegetable fat (HVF) in sickness behavior induced by LPS in aged mice. Male C57BJ/6 aged mice received a supplementation with HVF for six months. After HVF supplementation mice were treated with LPS (0.15 mg/kg; i. p. injection). Twenty-four hours post LPS injection mice were submitted to behavioral tests and then, the hippocampus, striatum and prefrontal cortex were removed for neurochemical determinations. Our results showed that dietary HVF did not exacerbate the behavioral alterations induced by LPS. Although HVF did not modulate the proinflammatory cytokines analyzed, it caused a potentiation in the increase of brain tumor necrosis factor-alpha levels induced by LPS. Moreover, dietary HVF aggravated LPS-induced KP activation in the brain of mice, mainly by further increase of neurotoxic metabolite quinolinic acid and further decrease of kynurenic acid/kynurenine ratio, a marker of neuroprotective branch of KP. Overall, our study demonstrated that dietary HVF did not worsen the sickness behavioral induced by LPS administration. However, HVF aggravated the activation of KP and exacerbated the shift of KP metabolism towards the neurotoxic branch. |
Databáze: | OpenAIRE |
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