Seasonally variable intestinal metagenomes of the red palm weevil ( R hynchophorus ferrugineus )
| Autor: | Shangang Jia, Songnian Hu, An Yin, Mohammed Baabdullah, Fusen Li, Xiaowei Zhang, Duojun Zhao, Tala, Jun Yu, Xiaoguang Yu, Quanzheng Yun, Guangyu Zhang, Ibrahim S. Al-Mssallem, Sun Zhang, Lei Wang, Gaoyuan Sun, Jixiang Wang |
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| Rok vydání: | 2013 |
| Předmět: |
Glycoside Hydrolases
Biological pest control Zoology Euryarchaeota Gut flora Microbiology Polysaccharides RNA Ribosomal 16S Animals Research Articles Ecology Evolution Behavior and Systematics Base Sequence biology business.industry Host (biology) Ecology Weevil Phoeniceae Pest control Sequence Analysis DNA biology.organism_classification Intestines Lactococcus lactis Klebsiella pneumoniae Rhynchophorus Larva Metagenome Weevils Pyrosequencing Seasons PEST analysis business |
| Zdroj: | Environmental Microbiology |
| ISSN: | 1462-2920 1462-2912 |
| Popis: | The intestinal microbes residing in the red palm weevil (RPW, Rhynchophorus ferrugineus) larva consume tender interior fibrous tissues of date palm trunks. The understanding of such microbiota at molecular level provides vital clues for the biological control of this devastating pest. Using pyrosequencing and shotgun strategy, we first study taxonomic profiles of the microbiota sampled at different months (March, July and November), and then confirm the impact of high-temperature stress on the microbial populations based on data from 16S rRNA amplicons using both field and laboratory samples. We further identify Klebsiella pneumoniae in November and Lactococcus lactis in July as the dominant species of the microbiota. We find that the RPW gut microbiota degrades polysaccharides and sucrose with hydrolases and that different active bacterial species in November and July are responsible for the symbiotic relationship between the microbiota and the host. Our results provide vital information for pest control and cellulolytic bacterial species characterization. |
| Databáze: | OpenAIRE |
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