Synthesis of Nano-Paramagnetic Oleuropein to Induce KRAS Over-Expression: A New Mechanism to Inhibit AGS Cancer Cells
Autor: | Mohammad Zaefizadeh, Farhad Barzegar, Ali Salehzadeh, Reza Yari |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Medicine (General) Antioxidant medicine.medical_treatment Iridoid Glucosides Gene Expression Adenocarcinoma AGS Cancer K-Ras miR-200 oncogene nano-oleuropein oleuropein real-time PCR medicine.disease_cause Article Proto-Oncogene Proteins p21(ras) 03 medical and health sciences chemistry.chemical_compound Paramagnetism R5-920 0302 clinical medicine Stomach Neoplasms Oleuropein Olea medicine Humans Iridoids IC50 Cell Proliferation business.industry Spectrometry X-Ray Emission General Medicine Molecular biology 030104 developmental biology Real-time polymerase chain reaction chemistry Cell culture 030220 oncology & carcinogenesis Cancer cell Nanoparticles KRAS business |
Zdroj: | Medicina; Volume 55; Issue 7; Pages: 388 Medicina Volume 55 Issue 7 Medicina, Vol 55, Iss 7, p 388 (2019) |
ISSN: | 1648-9144 |
Popis: | Background and objectives: Human gastric adenocarcinoma (AGS) is one of the most common malignant cancers worldwide. The present study aimed to transfer oleuropein into cancer cells using synthetic paramagnetic nanoparticles and study their effect on the AGS (ATCC® CRL1739&trade ) cell line. Materials and Methods: Paramagnetic nano-oleuropein was synthesized using four-stage co-precipitation by developing NH-connected bridges and was evaluated by EDS, SEM and FTIR methods. Different concentrations of magnetic oleuropein (0, 0.15, 0.45, 1.37, 4.12, 12.35, 37.04, 111.11, 333.33, 1000 µ g/mL) were used to treat the AGS cell line in a completely randomized design using a statistical framework with three replicates. The relative expression rate of miR-200 and KRAS oncogenes was evaluated using real-time PCR. The inhibition rate of the AGS cells was assessed using the MTT test at 24, 48 and 72 h intervals. Results: The results showed that there was a significant difference between the inhibition rates of magnetic nano-oleuropein at IC50-24h (23.6 µ g/mL), IC50-48h (15.2 µ g/mL) and IC50-72h (9.2 µ g/mL). Real-time PCR indicated that the relative expression of KRAS and miR-200 genes was highest at IC50 at these intervals. Conclusions: Magnetic nano-oleuropein can be subjected to objective testing and clinical evaluations as a natural antioxidant to prevent and treat gastric adenocarcinoma. |
Databáze: | OpenAIRE |
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