Quantitative study of interaction of deacylated tRNA with Escherichia coli ribosomes Role of 50 S subunits in formation of the E site
| Autor: | Yu.P. Semenkov, Evgeny M. Makarov, S.V. Kirillov |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Protein subunit
Biophysics E-site Biology Biochemistry Ribosome RNA Transfer Antibiotic tetracycline edeine Structural Biology Genetics Escherichia coli Ribosome exit site Molecular Biology Binding Sites Edeine Translation (biology) Cell Biology Tetracycline TRNA binding Deacylated tRNA—ribosome interaction A-site Site distribution between subunits Transfer RNA T arm Ribosomes |
| Zdroj: | FEBS Letters. (1):91-94 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(83)81122-3 |
| Popis: | The 30 S subunit contains 2 sites for tRNA binding (Phe-tRNA, AcPhe-tRNA, tRNAPheOH) with the functional properties of D and A sites of the 70 S ribosome after attachment of 50 S subunit. The third (E) site specific for deacylated tRNA is introduced into 70 S ribosome by its 50 S subunit. The E-site binding of tRNAPheOH is not sensitive to either tetracycline and edeine, and practically codon-independent. The affinity constant of tRNAPheOH for the E site is 2–3 orders of magnitude lower than that for the D site. |
| Databáze: | OpenAIRE |
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