Serum activity of matrix metalloproteinase-2 and -9 is increased in chronic post-stroke individuals: a cross-sectional exploratory study
| Autor: | Jonathan Emanuel Cunha, Thiago Luiz Russo, Luisa Fernanda García-Salazar, Stela Márcia Mattiello, Jean Alex Matos Ribeiro |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Inflammation Atrophy Internal medicine medicine Gelatinase Humans Stroke Sedentary lifestyle Community and Home Care business.industry Interleukin-6 Tumor Necrosis Factor-alpha Rehabilitation medicine.disease Physical activity level Interleukin-10 Cross-Sectional Studies Matrix Metalloproteinase 9 Matrix Metalloproteinase 2 Neurology (clinical) medicine.symptom business Cadence Bioelectrical impedance analysis Biomarkers |
| Zdroj: | Topics in stroke rehabilitation. 29(8) |
| ISSN: | 1945-5119 |
| Popis: | Background High concentrations of matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) are associated with inflammation early after stroke. In chronic stages, the elevation of some cytokines is related to the presence of co-morbid conditions in these individuals. In addition to this, some characteristics such as sensorimotor impairment, atrophy, and sedentary lifestyle predispose the system to an inflammatory response. Objective To quantify MMP-2 and -9 serum activity in chronic post-stroke individuals and correlate it with variables of physical activity level, body composition, functional and walking capacity, and with inflammatory biomarkers. Additionally, gelatinase activity was characterized according to motor impairment. Methods Fourteen patients with stroke onset >6 months and seven healthy individuals were enrolled in this study. The clinical assessment included: body composition, measure by bioelectrical impedance analyzer; Fugl-Meyer Motor Assessment Scale; six-minute and ten-meter walk tests, and physical activity level assessed by the StepWatch® Activity Monitor. Blood samples were collected from antecubital vein and serum MMP-2 and -9 activity was analyzed using gelatin Zymography, and the TNFα, IL-6, IL-1β, IL-10 biomarkers using ELISA kits. Results Chronic post-stroke individuals presented an increased activity of MMP-2 and -9 compared to healthy individuals. Positive correlations with time and steps in low cadence and negative ones with medium cadence and peak activity index were observed. According to the motor impairment, the MMP-2 activity was increased in the mild-moderate group compared to the control group. Conclusion Increased gelatinases in chronic post-stroke individuals could describe an inflammation process related to the limited capacity of walking in high intensities. |
| Databáze: | OpenAIRE |
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