A novel family of calmodulin-binding transcription activators in multicellular organisms
| Autor: | Hillel Fromm, Ariel Scharlat, Nicolas Bouché, David Bouchez, Wayne A. Snedden |
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| Přispěvatelé: | Unité de recherche Génétique et amélioration des plantes (GAP), Institut National de la Recherche Agronomique (INRA), University of Leeds, Plant Science Department, University of Pretoria (UPSpace), Department of Biology, Universita degli Studi di Padova, School of Biology, Department of Plant Sciences, Tel Aviv University [Tel Aviv] |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Insecta
Transcription Genetic Amino Acid Motifs Arabidopsis membrane de plasma Immunoglobulin domain Biochemistry Chromatography Affinity régulation Transcription (biology) Cloning Molecular Peptide sequence Conserved Sequence Phylogeny Glutathione Transferase Genetics DOMAINE SITE Cell biology [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Databases as Topic Multigene Family plante Drosophila Repressor lexA expression des gènes Protein Binding Signal Transduction Transcriptional Activation DNA Complementary Calmodulin Molecular Sequence Data glutamate Biology Cell Line Two-Hybrid System Techniques décarboxylase Animals Humans Amino Acid Sequence camta Caenorhabditis elegans Molecular Biology Transcription factor Gene Gene Library Cell Nucleus calcium Dose-Response Relationship Drug Models Genetic Sequence Homology Amino Acid arabidopsis thaliana Cell Biology DNA beta-Galactosidase séquence de protéines Protein Structure Tertiary Kinetics biology.protein boucle Ankyrin repeat Peptides génome humain |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2002, 277 (24), pp.21851-21861. ⟨10.1074/jbc.M200268200⟩ Journal of Biological Chemistry 24 (277), 21851-21861. (2002) |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.M200268200⟩ |
| Popis: | Screening of cDNA expression libraries derived from plants exposed to stress, with35S-labeled recombinant calmodulin as a probe, revealed a new family of proteins containing a transcription activation domain and two types of DNA-binding domains designated the CG-1 domain and the transcription factor immunoglobulin domain, ankyrin repeats, and a varying number of IQ calmodulin-binding motifs. Based on domain organization and amino acid sequence comparisons, similar proteins, with the same domain organization, were identified in the genomes of other multicellular organisms including human,Drosophila, and Caenorhabditis, whereas none were found in the complete genomes of single cell eukaryotes and prokaryotes. This family of proteins was designated calmodulin-binding transcription activators (CAMTAs). Arabidopsis thalianacontains six CAMTA genes (AtCAMTA1–AtCAMTA6). The transcription activation domain of AtCAMTA1 was mapped by testing a series of protein fusions with the DNA-binding domain of the bacterial LexA transcription factor and two reporter genes fused to LexA recognition sequences in yeast cells. Two human proteins designated HsCAMTA1 and HsCAMTA2 were also shown to activate transcription in yeast using the same reporter system. Subcellular fractionation ofArabidopsis tissues revealed the presence of CAMTAs predominantly in the nucleus. Calmodulin binding assays identified a region of 25 amino acids capable of binding calmodulin with high affinity (K d = 1.2 nm) in the presence of calcium. We suggest that CAMTAs comprise a conserved family of transcription factors in a wide range of multicellular eukaryotes, which possibly respond to calcium signaling by direct binding of calmodulin. |
| Databáze: | OpenAIRE |
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