Laser-assisted cryosurgery in ex vivo mice hepatic tissue: viability assays using green fluorescent protein
| Autor: | L. Martínez-Suástegui, B. Duperray, Felipe Godinez, Gabriel Guillén, Adam Broadbent Slade, Guillermo Aguilar |
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| Rok vydání: | 2011 |
| Předmět: |
Cryoablation
Pathology medicine.medical_specialty Cell Survival medicine.medical_treatment Green Fluorescent Proteins H&E stain RT-PCR Biomedical Engineering Biology In Vitro Techniques Cryosurgery Medical and Health Sciences Article Fluorescence 030218 nuclear medicine & medical imaging Green fluorescent protein 03 medical and health sciences Mice 0302 clinical medicine Engineering medicine Animals Hepatectomy Viability assay Microscopy Prostate cancer Reverse transcription polymerase chain reaction Real-time polymerase chain reaction Treatment Outcome Microscopy Fluorescence Liver 030220 oncology & carcinogenesis Biophysics Laser Therapy Ex vivo |
| Zdroj: | Annals of biomedical engineering, vol 39, iss 2 Annals of Biomedical Engineering |
| Popis: | An experimental investigation is carried out to develop a novel approach to cryosurgery, where laser heating counteracts tissue freezing to better confine damage to the targeted cancerous tissue within a lethal low-temperature isothermal boundary—an approach we refer to as laser-assisted cryosurgery (LAC). The advantage of this procedure relative to conventional cryosurgery assisted with urethral warmers or cryoheaters is that laser heating provides volumetric rather than superficial heating, which leads to deeper penetration, more homogeneous tissue protection and better demarcation of the destructive freezing effect to a well-defined targeted volume. Tissue viability assays are performed using green fluorescence protein (GFP) as a viability marker and correlated with temperature history after performing LAC procedures on ex vivo mice hepatic tissue. The limit for cell denaturation at the irradiated surface predicted by GFP analysis is further confirmed using reverse transcription polymerase chain reaction (RT-PCR). In addition, the correlation between GFP fluorescence and cell viability and loss of GFP fluorescence in non-viable cells has been tested and validated by histological analysis using a standard cell viability measuring method (hematoxylin and eosin staining). Analysis of our experimental measurements show that reproducible thermal gradients (of 236 °C/cm) and predictable tissue necrosis can be reliably produced by LAC without exceeding temperature thresholds for cell denaturation (of T surf ≈ 48 °C) beyond preset tissue boundaries (with resolution of 0.1 °C/mm). The results have shown the feasibility of controlling temperatures at specified tissue locations to prevent hyperthermal or freezing damage. |
| Databáze: | OpenAIRE |
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