Negative feedback regulation of UV-B–induced photomorphogenesis and stress acclimation in Arabidopsis

Autor: Werner Heller, Andreas Albert, Marc Heijde, Roman Ulm, Harald K. Seidlitz, Henriette Gruber
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Morphogenesis/radiation effects
UVR8
Mutation/genetics
RNA
Messenger/genetics/metabolism
Ultraviolet Rays
Acclimatization
Arabidopsis
Repressor
Gene Expression Regulation
Plant
Stress
Physiological
Morphogenesis
RNA
Messenger
Transcription factor
Feedback
Physiological/radiation effects
Arabidopsis Proteins/genetics/metabolism
Genetics
Feedback
Physiological
Multidisciplinary
Arabidopsis/growth & development/radiation effects
biology
Signal Transduction/radiation effects
Abiotic stress
Arabidopsis Proteins
Acclimatization/radiation effects
Biological Sciences
biology.organism_classification
Ubiquitin ligase
Cell biology
ddc:580
Gene Expression Regulation
Plant/radiation effects
Stress
Physiological/radiation effects
Protein Binding/radiation effects
Photobiology
Mutation
biology.protein
Photomorphogenesis
Protein Binding
Signal Transduction
Zdroj: Proceedings of the National Academy of Sciences, Vol. 107, No 46 (2010) pp. 20132-20137
ISSN: 0027-8424
Popis: Plants respond to low levels of UV-B radiation with a coordinated photomorphogenic response that allows acclimation to this environmental stress factor. The key players in this UV-B response are COP1 (an E3 ubiquitin ligase), UVR8 (a β-propeller protein), and HY5 (a bZIP transcription factor). We have shown previously that an elevated UV-B–specific response is associated with dwarf growth, indicating the importance of balancing UV-B–specific signaling. Negative regulators of this pathway are not known, however. Here, we describe two highly related WD40-repeat proteins, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 (RUP1) and RUP2, that interact directly with UVR8 as potent repressors of UV-B signaling. Both genes were transcriptionally activated by UV-B in a COP1-, UVR8-, and HY5-dependent manner. rup1 rup2 double mutants showed an enhanced response to UV-B and elevated UV-B tolerance after acclimation. Overexpression of RUP2 resulted in reduced UV-B–induced photomorphogenesis and impaired acclimation, leading to hypersensitivity to UV-B stress. These results are consistent with an important regulatory role for RUP1 and RUP2, which act downstream of UVR8–COP1 in a negative feedback loop impinging on UVR8 function, balancing UV-B defense measures and plant growth.
Databáze: OpenAIRE
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