Comparative evaluation of aggressiveness traits in staphylococcal strains from severe infections versus nasopharyngeal carriage
| Autor: | Alina Cristina Neguţ, Irina Codiţă, Anca Streinu-Cercel, Ioana Berciu, Lilia Matei, Mariana Carmen Chifiriuc, Petre Iacob Calistru, Dan Oţelea, Adrian Streinu-Cercel, Olga Mihaela Dorobăţ, Daniel Pirici, Coralia Bleotu, Daniela Adriana Ion, Oana Săndulescu, Elena Carmina Drăgulescu, Alexandru Rafila, Mihaela Oprea, Daniela Tălăpan |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Adolescent Virulence Factors Staphylococcus Fulminant 030106 microbiology Virulence Comorbidity Microbial Sensitivity Tests Biology medicine.disease_cause Severity of Illness Index Microbiology Asymptomatic Bacterial Adhesion Cell Line Young Adult 03 medical and health sciences Quantitative Trait Heritable Nasopharynx medicine Humans Child Aged Aged 80 and over Infant Newborn Biofilm Genetic Variation Infant Adhesion Middle Aged Staphylococcal Infections Phenotype Anti-Bacterial Agents Infectious Diseases Susceptible individual Biofilms Child Preschool Carrier State Immunology Female medicine.symptom |
| Zdroj: | Microbial Pathogenesis. 102:45-53 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2016.11.006 |
| Popis: | Despite their commensal status, staphylococci can become problematic pathogens expressing multiple and redundant virulence factors. This study aimed to evaluate aggressiveness markers comparatively in staphylococcal strains isolated from severe infections versus asymptomatic carriage in order to identify clinically relevant bacterial traits that could easily be detected in clinical practice and could be suggestive for particular host-pathogen interactions such as cyto-adhesion or biofilm formation, ultimately orienting the clinical decision-making process. We have used in vitro phenotypic methods to assess adhesion to and invasion of eukaryotic cells, biofilm development, and expression of soluble virulence factors in 92 Staphylococcus spp. strains. The adhesion index, invasion capacity, biofilm formation and expression of soluble factors did not differ significantly between clinical and commensal strains. The major bacterial traits we found to be significantly more prevalent in clinical staphylococci were the aggregative adhesion pattern (P = 0.012), cluster adhesion (P = 0.001) and tetrad morphology (P = 0.018). The aggregative adhesion pattern was correlated with higher cyto-adhesion (P |
| Databáze: | OpenAIRE |
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