Simultaneous determination of proteins in microstructured optical fibers supported by chemometric tools
| Autor: | Natalia A. Burmistrova, Yulia B. Monakhova, Pavel S. Pidenko, Sergei A. Pidenko, Yulia S. Skibina |
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| Rok vydání: | 2019 |
| Předmět: |
Models
Molecular Optical fiber Ovalbumin 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry law.invention Chemometrics Mice law Partial least squares regression Calibration Animals Horses Bovine serum albumin Least-Squares Analysis Optical Fibers Chromatography biology Chemistry 010401 analytical chemistry Albumin Serum Albumin Bovine 021001 nanoscience & nanotechnology 0104 chemical sciences Immunoglobulin G biology.protein Cattle 0210 nano-technology Ternary operation Quantitative analysis (chemistry) Chickens Blood Chemical Analysis |
| Zdroj: | Analytical and bioanalytical chemistry. 411(27) |
| ISSN: | 1618-2650 |
| Popis: | A new perspective on the relevant problem—creating simple, rapid, and efficient protein sensors based on microstructured optical fibers using a simple homogeneous analysis format—was proposed. Commercially available long-period grating hollow core microstructured optical fibers (LPG HCMOF) were used to determine bovine serum albumin (BSA) and albumin from chicken eggs (OVA) in binary mixtures as well as immunoglobulin G (IgG) in the presence of BSA and OVA. LPG HCMOF transmission spectra allowed the detection of both BSA and OVA up to 10 mg/mL with LOD as low as 0.1 and 0.8 μg/mL, respectively. Partial least squares regression (PLS) was utilized for modeling of LPG HCMOF spectral data and quantitative analysis of BSA, OVA, total protein, and IgG in binary and ternary mixtures. Rather high coefficients of determination (R2) and low root mean square error for the calibration (RMSEC) (15%) and prediction (RMSEP) (20%) were obtained for all PLS models. The proposed approach was tested in the analysis of BSA in spiked horse blood hemolyzed (HBH). The results demonstrated the functionality of the proposed approach and offered the opportunity for the creation of a wide range of sensors for protein determination in complex mixtures. |
| Databáze: | OpenAIRE |
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