Salicylate and Procyanidin-Rich Stem Extracts of Gaultheria procumbens L. Inhibit Pro-Inflammatory Enzymes and Suppress Pro-Inflammatory and Pro-Oxidant Functions of Human Neutrophils Ex Vivo

Autor: Anna Magiera, Piotr Michel, Łukasz Poraj, Sebastian Granica, Małgorzata Jurek, Karolina Rosińska, Monika A. Olszewska
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Antioxidant
medicine.medical_treatment
Anti-Inflammatory Agents
antioxidant activity
Pharmacology
Catechin
lcsh:Chemistry
Lipoxygenase
chemistry.chemical_compound
0302 clinical medicine
neutrophils
Gaultheria procumbens
anti-inflammatory activity
lcsh:QH301-705.5
Spectroscopy
biology
Plant Stems
Chemistry
General Medicine
procyanidins
Computer Science Applications
Phytochemical
030220 oncology & carcinogenesis
Cytokines
Gaultheria
Adult
Cell Survival
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Young Adult
medicine
Biflavonoids
Humans
Proanthocyanidins
Physical and Theoretical Chemistry
oxidative burst
Molecular Biology
polyphenols
salicylates
elastase 2
Plant Extracts
Organic Chemistry
Pro-oxidant
In vitro
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Polyphenol
biology.protein
interleukin 1β
Reactive Oxygen Species
Ex vivo
Methyl salicylate
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 7
International Journal of Molecular Sciences, Vol 20, Iss 7, p 1753 (2019)
ISSN: 1422-0067
DOI: 10.3390/ijms20071753
Popis: Salicylate-rich plants are an attractive alternative to synthetic anti-inflammatory drugs due to a better safety profile and the advantage of complementary anti-inflammatory and antioxidant effects of the co-occurring non-salicylate phytochemicals. Here, the phytochemical value and biological effects in vitro and ex vivo of the stems of one of such plants, Gaultheria procumbens L., were evaluated. The best extrahent for effective recovery of the active stem molecules was established in comparative studies of five extracts. The UHPLC-PDA-ESI-MS3, HPLC-PDA, and UV-photometric assays revealed that the selected acetone extract (AE) accumulates a rich polyphenolic fraction (35 identified constituents
total content 427.2 mg/g dw), mainly flavanols (catechins and proanthocyanidins
201.3 mg/g dw) and methyl salicylate glycosides (199.9 mg/g dw). The extract and its model components were effective cyclooxygenase-2, lipoxygenase, and hyaluronidase inhibitors
exhibited strong antioxidant capacity in six non-cellular in vitro models (AE and procyanidins)
and also significantly and dose-dependently reduced the levels of reactive oxygen species (ROS), and the release of cytokines (IL-1&beta
IL-8, TNF-&alpha
) and proteinases (elastase-2, metalloproteinase-9) in human neutrophils stimulated ex vivo by lipopolysaccharide (LPS) and N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP). The cellular safety of AE was demonstrated by flow cytometry. The results support the application of the plant in traditional medicine and encourage the use of AE for development of new therapeutic agents.
Databáze: OpenAIRE
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