Genome-wide comparisons of gene expression in adult versus elderly burn patients

Autor: Saeid Amini-Nik, Miliana Vojvodic, Ronald G. Tompkins, Marc G. Jeschke, Stephanie C. Dreckmann
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Oncology
Male
Critical Care and Emergency Medicine
Poison control
Gene Expression
Geriatric Depression
Transcriptome
0302 clinical medicine
Elderly
Medicine and Health Sciences
Gene Regulatory Networks
Young adult
Immune Response
Trauma Medicine
Geriatrics
Burn Management
Multidisciplinary
Depression
Age Factors
Genomics
Middle Aged
3. Good health
Exact test
Medicine
Female
Burns
Traumatic Injury
Transcriptome Analysis
Signal Transduction
Research Article
Adult
medicine.medical_specialty
Adolescent
Science
Geriatric Psychiatry
Immunology
Dermatology
03 medical and health sciences
Young Adult
Immune system
Internal medicine
Mental Health and Psychiatry
medicine
Genetics
Humans
Aged
business.industry
Genome
Human
Mood Disorders
Gene Expression Profiling
Biology and Life Sciences
Computational Biology
030208 emergency & critical care medicine
Genome Analysis
Gene expression profiling
030104 developmental biology
Gene Expression Regulation
Age Groups
People and Places
Population Groupings
business
Total body surface area
Biomarkers
Zdroj: PLoS ONE, Vol 14, Iss 12, p e0226425 (2019)
PLoS ONE
ISSN: 1932-6203
Popis: PurposeMortality and morbidity rates of elderly burn patients remain high despite numerous advancements in modern burn care. While prior studies have offered first insights on the biochemical changes in elderly burn patients compared to adults, the underlying cellular responses remain largely unknown. In this study, we aim to characterize the transcriptome of elderly burn patients and compare it to adult burn patients to obtain insights into the underlying molecular responses post-burn and to elucidate the effect of advanced age on the acute burn response.Materials and methodsMicroarray data obtained from the Glue Grant Trauma-Related Database was obtained from blood specimens for ten elderly patients (n = 10), each with a set of two sex and total body surface area (TBSA) matched adult controls (n = 20), during the acute phase post-burn. Adult and elderly demographics and clinical outcomes were contrasted using using the Chi-Square test, Fisher's Exact Test, or two-sample t-tests, as appropriate (pResultsSupervised analysis identified multiple genes that were differentially expressed between the elderly and adult groups. Pathway analysis and heatmap generation suggest that elderly patients share a distinct hypo-inflammatory response in the acute post-burn phase with downregulation of a number of immune-related pathways, including those related to antigen processing, specifically via MHC class I, ubiquitination and proteasome degradation (pConclusionsThe altered inflammatory and immune responses at the transcriptome level in elderly patients after burn are indicative of a failure in elderly burn patients to initiate an appropriate inflammatory and stress response during the acute phase post-burn.
Databáze: OpenAIRE
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