Cutting Unfixed Frozen Sections for Fluorescence Antibody Studies

Autor:	Louis Cj
Rok vydání:	1957
Předmět:	Pathology medicine.medical_specialty Frozen section procedure Histology Histological Techniques Immunologic Tests Fluorescence law.invention Isopentane chemistry.chemical_compound chemistry law Microtome medicine Frozen Sections Humans Anatomy Frozen tissue Biomedical engineering
Zdroj:	Stain Technology. 32:279-282
ISSN:	0038-9153
DOI:	10.3109/10520295709111640
Popis:	A simplified method of section cutting, dispensing with guide attachment on microtome blade, and suitable for serial sections of unfixed frozen tissue of 4μ is described. Essential features are low temperature maintenance (—13°C), critical angle of knife and moistening of slides in cold alcohol or isopentane. This method has been found suitable for fluorescence antibody studies where maintenance of low temperatures is necessary.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::725dde57e98fbb16be49ec373aae3c76 https://doi.org/10.3109/10520295709111640 Zobrazit plný text záznamu