Degradation of 4-Fluorobiphenyl by Mycorrhizal Fungi as Determined by 19 F Nuclear Magnetic Resonance Spectroscopy and 14 C Radiolabelling Analysis
| Autor: | C. Till, J. Troke, N. A. Green, Jeremy K. Nicholson, Andrew A. Meharg |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Biphenyl
Magnetic Resonance Spectroscopy Ecology biology Chemistry Basidiomycota Biphenyl Compounds Fluorine-19 NMR Nuclear magnetic resonance spectroscopy biology.organism_classification Applied Microbiology and Biotechnology Biphenyl compound NMR spectra database chemistry.chemical_compound Environmental and Public Health Microbiology Biodegradation Environmental Biotransformation Organic chemistry Carbon Radioisotopes Microbial biodegradation Mycorrhiza Chromatography High Pressure Liquid Food Science Biotechnology |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.65.9.4021-4027.1999 |
| Popis: | The pathways of biotransformation of 4-fluorobiphenyl (4FBP) by the ectomycorrhizal fungus Tylospora fibrilosa and several other mycorrhizal fungi were investigated by using 19 F nuclear magnetic resonance (NMR) spectroscopy in combination with 14 C radioisotope-detected high-performance liquid chromatography ( 14 C-HPLC). Under the conditions used in this study T. fibrillosa and some other species degraded 4FBP. 14 C-HPLC profiles indicated that there were four major biotransformation products, whereas 19 F NMR showed that there were six major fluorine-containing products. We confirmed that 4-fluorobiphen-4′-ol and 4-fluorobiphen-3′-ol were two of the major products formed, but no other products were conclusively identified. There was no evidence for the expected biotransformation pathway (namely, meta cleavage of the less halogenated ring), as none of the expected products of this route were found. To the best of our knowledge, this is the first report describing intermediates formed during mycorrhizal degradation of halogenated biphenyls. |
| Databáze: | OpenAIRE |
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