Detection of Nipah virus in Pteropus medius in 2019 outbreak from Ernakulam district, Kerala, India
| Autor: | A P Sugunan, Rima R Sahay, Triparna D. Majumdar, Dimpal A Nyayanit, M D Gokhale, Dilip R. Patil, Savita Patil, Rajlaxmi Jain, Nivedita Gupta, Sanjay Gopale, Anita M. Shete, Devendra T. Mourya, A B Sudeep, R. Balasubramanian, Prachi G. Pardeshi, Pragya D Yadav |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Veterinary medicine Nipah virus 030106 microbiology India Ernakulum Biology Antibodies Viral Real-Time Polymerase Chain Reaction Disease Outbreaks lcsh:Infectious and parasitic diseases 03 medical and health sciences Medical microbiology Chiroptera Genotype medicine Animals lcsh:RC109-216 Index case Phylogeny Henipavirus Infections IgG ELISA Rectum High-Throughput Nucleotide Sequencing Outbreak Pteropus medius Pteropus biology.organism_classification 030104 developmental biology Infectious Diseases Parasitology Immunoglobulin G NGS RNA Viral Pteropus spp Research Article |
| Zdroj: | BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-7 (2021) BMC Infectious Diseases |
| ISSN: | 1471-2334 |
| Popis: | Background In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month. Methods Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats. Results One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats. Conclusion A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection. |
| Databáze: | OpenAIRE |
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