Antimicrobial Activity and Probable Mechanisms of Action of Medicinal Plants of Kenya: Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus
| Autor: | Esther N. Matu, Peter Amwoga Ayeka, Joyce Ondicho, Christine Bii, Peter Mwitari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Bacterial Diseases
lcsh:Medicine Gene Expression Global Health Mice Anti-Infective Agents Agar Medicinal plants lcsh:Science Multidisciplinary Withania Antimicrobial Fungicide Infectious Diseases Prunus africana Cytokines Medicine Research Article food.ingredient Immunology Microbial Sensitivity Tests Biology Withania somnifera Microbiology Cell Line Molecular Genetics food Complementary and Alternative Medicine Genetics Animals Agar diffusion test Cell Proliferation Plants Medicinal Bacteria Plant Extracts Interleukin-7 lcsh:R Fungi Computational Biology Molecular Development biology.organism_classification Kenya McFarland standards Tracheophyta Gene Expression Regulation lcsh:Q Medicine Traditional Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 6, p e65619 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus are used traditionally in Kenya for treatment of microbial infections and cancer. Information on their use is available, but scientific data on their bioactivity, safety and mechanisms of action is still scanty. A study was conducted on the effect of organic extracts of these plants on both bacterial and fungal strains, and their mechanisms of action. Extracts were evaluated through the disc diffusion assay. Bacteria and yeast test strains were cultured on Mueller-Hinton agar and on Sabouraud dextrose agar for the filamentous fungi. A 0.5 McFarland standard suspension was prepared. Sterile paper discs 6 mm in diameter impregnated with 10 µl of the test extract (100 mg/ml) were aseptically placed onto the surface of the inoculated media. Chloramphenicol (30 µg) and fluconazole (25 µg) were used as standards. Discs impregnated with dissolution medium were used as controls. Activity of the extracts was expressed according to zone of inhibition diameter. MIC was determined at 0.78-100 mg/ml. Safety studies were carried using Cell Counting Kit 8 cell proliferation assay protocol. To evaluate extracts mechanisms of action, IEC-6 cells and RT-PCR technique was employed in vitro to evaluate Interleukin 7 cytokine. Investigated plants extracts have both bactericidal and fungicidal activity. W. ugandensis is cytotoxic at IC50200 µg/ml). Fractions from W. ugandensis and W. somnifera too demonstrated antimicrobial activity. Mechanisms of action can largely be attributed to cytotoxicity, Gene silencing and immunopotentiation. Use of medicinal plants in traditional medicine has been justified and possible mechanisms of action demonstrated. Studies to isolate and characterize the bioactive constituents continue. |
| Databáze: | OpenAIRE |
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