A systematic approach to isomorphous replacement using a series of simple charged mercurials

Autor:	Daniel S.C. Yang, Neil B. Madsen, R.J. Fletterick, Stephen G. Withers
Rok vydání:	1979
Předmět:	Organomercury Compounds Protein Conformation Chemistry Biophysics Cell Biology Biochemistry Glycogen phosphorylase Crystallography X-Ray Diffraction Animals Molecule Phosphorylase a Rabbits Binding site Protein crystallization Molecular Biology
Zdroj:	Biochemical and Biophysical Research Communications. 86:1044-1050
ISSN:	0006-291X
DOI:	10.1016/0006-291x(79)90222-5
Popis:	Three novel mercury derivatives bearing different electronic charges have been synthesized and used to prepare useful heavy atom derivatives of crystals of glycogen phosphorylase a . While exhibiting some common binding sites on the exterior of the molecule, their internal binding sites are determined primarily by their charge as predicted. Their ease of preparation and general applicability makes them useful candidates for the preparation of heavy atom derivatives of any protein crystals in X-ray crystallographic studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7255ec539468e9ff1e726397ff18eecf https://doi.org/10.1016/0006-291x(79)90222-5 Zobrazit plný text záznamu