Microbial Community Structure Along a Horizontal Oxygen Gradient in a Costa Rican Volcanic Influenced Acid Rock Drainage System
| Autor: | Fernando Puente-Sánchez, Eduardo Libby, Alejandro Arce-Rodríguez, Dietmar H. Pieper, Raúl Mora-Amador, Michael E. Martinez, Roberto Avendaño, Max Chavarría, Keilor Rojas-Jimenez |
|---|---|
| Přispěvatelé: | Universidad de Costa Rica, Ministerio de Ciencia, Tecnología y Telecomunicaciones (Costa Rica), Federal Ministry of Education and Research (Germany), European Research Council, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Costa Rica 030106 microbiology Soil Science Microbial communities RNA Archaeal Volcanic Eruptions engineering.material Biology 03 medical and health sciences chemistry.chemical_compound Microbial ecology Rivers Oxygen gradient Oxigen gradient RNA Ribosomal 16S Ecosystem Sulfate Ecology Evolution Behavior and Systematics Ecology Bacteria Acidocella Microbiota Hydrogen-Ion Concentration biology.organism_classification Archaea Acid rock drainage Oxygen RNA Bacterial 030104 developmental biology Thermoplasmatales Microbial population biology chemistry Environmental chemistry engineering Pyrite San Cayetano |
| Zdroj: | Repositorio UNA Universidad Nacional de Costa Rica instacron:UNA Microbial ecology United States Microbial Ecology volume 80, pages793–808(2020) Kérwá Universidad de Costa Rica instacron:UCR Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | We describe the geochemistry and microbial diversity of a pristine environment that resembles an acid rock drainage (ARD) but it is actually the result of hydrothermal and volcanic influences. We designate this environment, and other comparable sites, as volcanic influenced acid rock drainage (VARD) systems. The metal content and sulfuric acid in this ecosystem stem from the volcanic milieu and not from the product of pyrite oxidation. Based on the analysis of 16S rRNA gene amplicons, we report the microbial community structure in the pristine San Cayetano Costa Rican VARD environment (pH = 2.94–3.06, sulfate ~ 0.87–1.19 g L−1, iron ~ 35–61 mg L−1 (waters), and ~ 8–293 g kg−1 (sediments)). San Cayetano was found to be dominated by microorganisms involved in the geochemical cycling of iron, sulfur, and nitrogen; however, the identity and abundance of the species changed with the oxygen content (0.40–6.06 mg L−1) along the river course. The hypoxic source of San Cayetano is dominated by a putative anaerobic sulfate-reducing Deltaproteobacterium. Sulfur-oxidizing bacteria such as Acidithiobacillus or Sulfobacillus are found in smaller proportions with respect to typical ARD. In the oxic downstream, we identified aerobic iron-oxidizers (Leptospirillum, Acidithrix, Ferrovum) and heterotrophic bacteria (Burkholderiaceae bacterium, Trichococcus, Acidocella). Thermoplasmatales archaea closely related to environmental phylotypes found in other ARD niches were also observed throughout the entire ecosystem. Overall, our study shows the differences and similarities in the diversity and distribution of the microbial communities between an ARD and a VARD system at the source and along the oxygen gradient that establishes on the course of the river. This work was supported by The Vice-rectory of Research of Universidad de Costa Rica (project number VI 809-B6-524), the Costa Rican Ministry of Science, Technology and Telecommunication (MICITT) and Federal Ministry of Education and Research (BMBF) (project VolcanZyme contract No FI-255B-17), and the ERC grant IPBSL (ERC250350-IPBSL). MM acknowledges government funding from the Transitorio I of the National Law 8488 for Emergencies and Risk Prevention in Costa Rica. F.P-S. is supported by grant IJC2018-035180-I from the Spanish Ministry of Science and Innovation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|