Pharmacological approach to increasing the retention of radiation-induced γ-H2AX foci using phosphatase inhibitors: significance in radiation biodosimetry
| Autor: | Kamalesh Dattaram Mumbrekar, B. S. Satish Rao, Akshaykumar A Nayak |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.diagnostic_test
Chemistry Phosphatase Public Health Environmental and Occupational Health General Medicine Protein phosphatase 2 Pharmacology In vitro 030218 nuclear medicine & medical imaging Flow cytometry 03 medical and health sciences 0302 clinical medicine Biodosimetry 030220 oncology & carcinogenesis Fluorescence microscope medicine Biomarker (medicine) Fostriecin Waste Management and Disposal |
| Zdroj: | Journal of Radiological Protection. 38:318-328 |
| ISSN: | 1361-6498 0952-4746 |
| DOI: | 10.1088/1361-6498/aaa97a |
| Popis: | In a scenario of accidental mass radiation exposure transportation and analysis of samples may take some time, resulting in loss of biomarker information over this period. The present study aims to use phosphatase inhibitors for longer retention of focal signals to adopt γ-H2AX as a biodosimetric biomarker for the management of early triage. Peripheral blood lymphocytes isolated from healthy individuals were irradiated in vitro with x-rays and γ-H2AX foci were analysed using fluorescent microscopy and flow cytometric methods. Further, the effect of protein phosphatase 2A inhibitors such as calyculin A, fostriecin and okadiac acid on the retention of foci was studied. Fluorescent microscopy was found to be a more sensitive method than flow cytometry. Calyculin A showed significant retention of focal signals at 6 h with 1.5-fold increased retention compared to radiation alone; this may prove beneficial in early triage management because of a better dose approximation. |
| Databáze: | OpenAIRE |
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