The effect of ferricyanide with iodoacetate in calcium-free solution on passive cation permeability in human red blood cells: comparison with the Gardos-effect and with the influence of PCMBS on passive cation permeability
| Autor: | G.F. Fuhrmann, Philip A. Knauf, H. Schneider, R. Fehlau |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Quinidine
Cell Membrane Permeability Potassium Channels Sodium Inorganic chemistry Biophysics chemistry.chemical_element Iodoacetates Calcium Biochemistry Sodium Channels chemistry.chemical_compound medicine Humans Ferricyanides Calcimycin Ion transporter Erythrocyte Membrane Cell Biology Phenylmercury Compounds Iodoacetic Acid Solutions EGTA Red blood cell medicine.anatomical_structure chemistry Quinacrine Permeability (electromagnetism) Ferricyanide Vanadates 4-Chloromercuribenzenesulfonate medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 983:179-185 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(89)90231-9 |
| Popis: | Freshly prepared human red blood cells incubated with 5 mM ferricyanide, 0.2 mM iodoacetate and 2 mM adenosine in the presence of 5 mM EGTA demonstrate comparable increases in Na+ and K+ permeability (ferricyanide effect). This effect is unrelated to the Ca2+-activated K+ channel (Gardos effect) since influx of Ca2+ from outside the cell is excluded. Also this effect is different from the non-specific Na+ and K+ permeability change elicited by PCMBS. These differences become obvious by using various reagents. For example, A23187 and quinidine exert opposite effects in Gardos and ferricyanide experiments, where A23187 and atebrin react oppositely in the latter and in PCMBS experiments. The ferricyanide effect described here does not involve formation of nonspecific channels. The change in Na+ permeability separately from K+ permeability under certain circumstances suggests a more specific effect. |
| Databáze: | OpenAIRE |
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