RAST-detection of specific IgE in diphenylmethane diisocyanate exposed workers: considerations in performance of the test
| Autor: | P. Germano, Angela Pezzini, A. Spiazzi, Paola Boccagni |
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| Rok vydání: | 1991 |
| Předmět: |
Time Factors
Immunology Serum albumin Diphenylmethane Ligands Immunoglobulin E Sensitivity and Specificity chemistry.chemical_compound Radioallergosorbent Test otorhinolaryngologic diseases medicine Humans Immunology and Allergy Incubation Cyanates Serum Albumin Chromatography biology medicine.diagnostic_test Chemistry Ligand Radioallergosorbent test Asthma Occupational Diseases Titer biology.protein Hapten Isocyanates |
| Zdroj: | Allergy. 46:166-172 |
| ISSN: | 1398-9995 0105-4538 |
| DOI: | 10.1111/j.1398-9995.1991.tb00565.x |
| Popis: | Some parameters which might affect RAST results, i.e. incubation length, degree of ligand substitution, and total IgE levels, were examined in a RAST system employing diphenylmethane diisocyanate--human serum albumin (MDI-HSA) conjugates as antigens, both in exposed symptomatic and in non-exposed subjects. The reaction equilibrium was reached after 9 and 18 h. respectively, in the first and the second step of the test. A marked correlation with the degree of ligand substitution was observed, the highest sensitivity being achieved with the lowest (2.9) MDI/HSA molar ratio examined. Higher (up to 50) degrees of substitution resulted in progressively lower levels of radioactivity bound and in a loss of specificity, as confirmed by RAST inhibition experiments. At high levels of ligand substitution, high titers of total serum IgE affected RAST results. |
| Databáze: | OpenAIRE |
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